Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.6.2.2 extracted from

  • Fujimoto, Y.; Shirabe, K.; Nagai, T.; Yubisui, T.; Takeshita, M.
    Role of Lys-110 of human NADH-cytochrome b5 reductase in NADH binding as probed by site-directed mutagenesis (1993), FEBS Lett., 322, 30-32.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type, K110A, K110M and K110R mutant enzymes in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
K110A 200fold elevated Km value for NADH, 85% of kcat Homo sapiens
K110M 1120fold elevated Km value for NADH Homo sapiens
K110R similar kinetic properties as wild-type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
NADH recombinant wild-type enzyme Homo sapiens
0.0023
-
NADH recombinant K110R mutant enzyme Homo sapiens
0.0066
-
ferricytochrome b5 recombinant wild-type enzyme Homo sapiens
0.0142
-
ferricytochrome b5 recombinant K110A mutant enzyme Homo sapiens
0.0167
-
ferricytochrome b5 recombinant K110R mutant enzyme Homo sapiens
0.0285
-
ferricytochrome b5 recombinant K110M mutant enzyme Homo sapiens
0.121
-
NADH recombinant K110A mutant enzyme Homo sapiens
0.672
-
NADH recombinant K110M mutant enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ferricytochrome b5 + NADH Homo sapiens enzyme participates in methemoglobin reduction in erythrocytes, in other tissues it plays a role in elongation and desaturation of fatty acids, P-450 mediated drug metabolism and cholesterol biosynthesis as part of the microsomal electron transfer system 2 ferrocytochrome b5 + NAD+ + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type, K110A, K110M and K110R mutant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome b5 + NADH enzyme participates in methemoglobin reduction in erythrocytes, in other tissues it plays a role in elongation and desaturation of fatty acids, P-450 mediated drug metabolism and cholesterol biosynthesis as part of the microsomal electron transfer system Homo sapiens 2 ferrocytochrome b5 + NAD+ + H+
-
?
NADH + ferricytochrome b5
-
Homo sapiens NAD+ + H+ + ferrocytochrome b5
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
563
-
ferricytochrome b5 recombinant K110M mutant enzyme Homo sapiens
742
-
ferricytochrome b5 recombinant K110A mutant enzyme Homo sapiens
872
-
ferricytochrome b5 recombinant wild-type enzyme Homo sapiens
877
-
ferricytochrome b5 recombinant K110R mutant enzyme Homo sapiens