General Stability | Organism |
---|---|
0.2% phosphatidycholine liposomes or Triton X-100 stabilize | Oryctolagus cuniculus |
freezing/thawing: 50% inactivation | Oryctolagus cuniculus |
Triton X-100 stabilizes | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Oryctolagus cuniculus | 5783 | - |
membrane | enzyme binds in vitro to a variety of membrane preparations, protease treatment removes a hydrophobic segment that is responsible for membrane binding | Oryctolagus cuniculus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE, gel filtration in the presence of 6 M guanidine hydrochloride, gel filtration in the presence of 0.5% sodium deoxycholate | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
622.5 | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-70°C, concentrated solution, several months, no loss of activity | Oryctolagus cuniculus |
-70°C, several months, no loss of activity | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome b5 + NADH | low activity with trypsin-solubilized cytochrome b5 | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
2 ferricytochrome b5 + NADH | specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
NADH + ferricyanide | - |
Oryctolagus cuniculus | NAD+ + H+ + ferrocyanide | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE, gel filtration in the presence of 6 M guanidine hydrochloride, gel filtration in the presence of 0.5% sodium deoxycholate | Oryctolagus cuniculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
667 | - |
ferricyanide | - |
Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Oryctolagus cuniculus |