Any feedback?
Literature summary for 1.6.2.2 extracted from
- Microsomal NADH-cytochrome b5 reductase of bovine brain: purification and properties (1983), J. Biochem., 94, 1547-1555.
General Stability
| General Stability | Organism |
|---|---|
| inactivation during dialysis is prevented by NADH | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Acrynol | 0.1 mM, 75% inhibition | Bos taurus |  |
| adenine nucleotides | - |
Bos taurus | |
| Atebrin | 1 mM, complete inhibition | Bos taurus | |
| Cl- | - |
Bos taurus | |
| iodoacetic acid | 5 mM, complete inhibition | Bos taurus | |
| N-ethylmaleimide | 10 mM, 89% inhibition | Bos taurus | |
| p-chloromercuribenzoate | 0.001 mM, complete inhibition | Bos taurus | |
| phosphate | competitive inhibition | Bos taurus | |
| Proflavin | 0.1 mM, 86% inhibition | Bos taurus | |
| taurodeoxycholate | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
NADH | - |
Bos taurus | |
| 0.0042 | - |
ferricyanide | - |
Bos taurus | |
| 0.0143 | - |
ferricytochrome b5 | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Bos taurus | 5783 | - |
| membrane | membrane-binding domain resides in the NH2-terminal region | Bos taurus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 31000, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| lysosome-solubilization | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 137 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, at least 1 week | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome b5 + NADH | specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol | Bos taurus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| 2 ferricytochrome b5 + NADH | no acceptors: ubiquinone-30, menadione, dihydrofolate, lipoamide | Bos taurus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| 2 ferricytochrome b5 + NADH | poor donor: NADPH | Bos taurus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| NADH + ferricytochrome b5 | - |
Bos taurus | NAD+ + H+ + ferrocytochrome b5 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, SDS-PAGE | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Bos taurus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
