Cloned (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U195 and Q8U194 | Q8U195: subunit alpha, Q8U194: subunit beta | - |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + 2 NADPH + 2 oxidized ferredoxin [iron-sulfur] cluster | reduction of both NAD+ and Fd by Nfn enzymes is coupled to the bifurcation of electrons from NADPH oxidation. The exergonic coupling of the NADP(H) and NAD(H) half reactions in Nfn is presumed to be mediated by NADPH oxidation at L-FAD followed by electron transfer to the proximal [2Fe-2S] cluster and subsequently the NAD(H) binding site at S-FAD. Biophysical analysis of the enzyme provides the first direct insight into the mechanism of flavin-based electron bifurcation and the requisite structural features | Pyrococcus furiosus | NADH + H+ + 2 NADP+ + 2 reduced ferredoxin [iron-sulfur] cluster | - |
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Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 31000 + 1 * 53000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
NfnI | - |
Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the 31000 Da subunit contains one FAD (S-FAD) and a [2Fe-2S] cluster with an unusual Asp ligand. The 53000 Da subunit contains one FAD (L-FAD), which is the site of electron bifurcation, and two [4Fe-4S] clusters. The L-FAD proximal [4Fe-4S] cluster coordination includes an unusual Glu ligand. S-FAD and L-FAD bind NADH and NADPH, respectively | Pyrococcus furiosus | |
[2Fe-2S]-center | the 31000 Da subunit contains one FAD (S-FAD) and a [2Fe-2S] cluster. The [2Fe-2S] cluster is unusual, with a relatively high (positive) reduction potential and coordination by one Asp and three Cys ligands | Pyrococcus furiosus | |
[4Fe-4S]-center | the 53000 Da subunit contains one FAD (L-FAD), which is the site of electron bifurcation, and two [4Fe-4S] clusters | Pyrococcus furiosus |