Literature summary for 1.6.1.4 extracted from

Nguyen, D.M.N.; Schut, G.J.; Zadvornyy, O.A.; Tokmina-Lukaszewska, M.; Poudel, S.; Lipscomb, G.L.; Adams, L.A.; Dinsmore, J.T.; Nixon, W.J.; Boyd, E.S.; Bothner, B.; Peters, J.W.; Adams, M.W.W.
Two functionally distinct NADP+-dependent ferredoxin oxidoreductases maintain the primary redox balance of Pyrococcus furiosus (2017), J. Biol. Chem., 292, 14603-14616 .

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Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	Q8U195 and Q8U194	Q8U195: subunit alpha, Q8U194: subunit beta	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin [iron-sulfur] cluster	-	Pyrococcus furiosus	NAD+ + 2 NADPH + 2 oxidized ferredoxin [iron-sulfur] cluster	-	?

Synonyms

Synonyms	Comment	Organism
NADH-dependent ferredoxin NADP+ oxidoreductase I	-	Pyrococcus furiosus
Nfn1	-	Pyrococcus furiosus
PF1327-28	-	Pyrococcus furiosus

Cofactor

Cofactor	Comment	Organism	Structure
flavin	flavoprotein	Pyrococcus furiosus

General Information

General Information	Comment	Organism
metabolism	the enzyme has a key role in maintaining redox homeostasis. It is involved in maintaining the cellular redox balance, producing NADPH for biosynthesis by recycling the two other primary redox carriers, NADH and ferredoxin. The enzyme effectively couples the endergonic reduction of NADP+ by NADH and the exergonic reduction of NADP+ by reduced ferredoxin, thereby maintaining a high ratio of NADPH/NADP+ to drive biosynthesis. The Pyrococcus furiosus genome encodes an NfnI paralog termed NfnII, and the two are differentially expressed, depending on the growth conditions. Deletion of the genes encoding either NfnI or NfnII affects the cellular concentrations of NAD(P)H and particularly NADPH. This results in a moderate to severe growth phenotype in deletion mutants, demonstrating a key role for each enzyme in maintaining redox homeostasis	Pyrococcus furiosus

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Release 2023.1 (January 2023)