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Literature summary for 1.6.1.4 extracted from

  • Wang, S.; Huang, H.; Moll, J.; Thauer, R.K.
    NADP+ reduction with reduced ferredoxin and NADP+ reduction with NADH are coupled via an electron-bifurcating enzyme complex in Clostridium kluyveri (2010), J. Bacteriol., 192, 5115-5123.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clostridium kluyveri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
NADPH pH 7, 37°C Clostridium kluyveri
1
-
NAD+ pH 7, 37°C Clostridium kluyveri

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Clostridium kluyveri 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster Clostridium kluyveri the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster
-
r
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster Clostridium kluyveri DSM 555 the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster
-
r

Organism

Organism UniProt Comment Textmining
Clostridium kluyveri A5N5D2 and A5N5D3 A5N5D2: NfnA, A5N5D3: NfnB
-
Clostridium kluyveri DSM 555 A5N5D2 and A5N5D3 A5N5D2: NfnA, A5N5D3: NfnB
-

Purification (Commentary)

Purification (Comment) Organism
-
Clostridium kluyveri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster in the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+). In the absence of ferredoxin, NAD+ is only very slowly reduced (less than 1% of the rate observed in the presence of ferredoxin) Clostridium kluyveri NADH + H+ + 2 NADP+ + reduced ferredoxin iron-sulfur cluster
-
r
NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster in the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+). In the absence of ferredoxin, NAD+ is only very slowly reduced (less than 1% of the rate observed in the presence of ferredoxin) Clostridium kluyveri DSM 555 NADH + H+ + 2 NADP+ + reduced ferredoxin iron-sulfur cluster
-
r
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH Clostridium kluyveri NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster
-
r
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH Clostridium kluyveri DSM 555 NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster
-
r
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH. In the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+) Clostridium kluyveri NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster
-
r
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH. In the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+) Clostridium kluyveri DSM 555 NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster
-
r

Subunits

Subunits Comment Organism
heterodimer
-
Clostridium kluyveri

Synonyms

Synonyms Comment Organism
NADH-dependent reduced ferredoxin:NADP+ oxidoreductase
-
Clostridium kluyveri
NfnAB
-
Clostridium kluyveri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Clostridium kluyveri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Clostridium kluyveri

Cofactor

Cofactor Comment Organism Structure
FAD iron-sulfur flavoprotein complex. Subunit NfnB contains FAD Clostridium kluyveri
iron-sulfur centre iron-sulfur flavoprotein complex, subunit NfnA has a predicted [2Fe2S] binding site, subunit NfnB has two predicted [4Fe4S] binding sites. UV-visible spectrum shows that the NfnAB complex contains up to 10 Fe molecules per heterodimer, which is consistent with the presence of two [4Fe4S] and one [2Fe2S] clusters Clostridium kluyveri