Literature summary for 1.6.1.2 extracted from

Bizouarn, T.; Grimley, R.; Diggle, C.; Thomas, C.M.; Jackson, J.B.
Mutations at tyrosine-235 in the mobile loop region of domain I protein of transhydrogenase from Rhodospirillum rubrum strongly inhibit hydride transfer (1997), Biochim. Biophys. Acta, 1320, 265-274.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of domain I Y235N and Y235F mutants in Escherichia coli	Rhodospirillum rubrum

Protein Variants

Protein Variants	Comment	Organism
Y235F	mutant domain I/wild-type domain III mixtures catalyse acetylpyridine adenine dinucleotide reduction with similar rates as wild-type domain I/wild-type domain III mixtures	Rhodospirillum rubrum
Y235N	mutant domain I/wild-type domain III mixtures catalyse acetylpyridine adenine dinucleotide reduction with similar rates as wild-type domain I/wild-type domain III mixtures	Rhodospirillum rubrum

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-
Rhodospirillum rubrum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADPH + oxidized 3-acetylpyridine adenine dinucleotide	enzyme also catalyzes a rapid, so called cyclic reaction, i.e. the reduction of acetylpyridine adenine dinucleotide in the presence of either NADP+ or NADPH: the NADPH/NADP+ remain permanently bound to domain III and are alternately oxidized by acetylpyridine adenine dinucleotide and then reduced by NADH in domain I	Bos taurus	NADP+ + reduced 3-acetylpyridine adenine dinucleotide	-	?
NADPH + oxidized 3-acetylpyridine adenine dinucleotide	enzyme also catalyzes a rapid, so called cyclic reaction, i.e. the reduction of acetylpyridine adenine dinucleotide in the presence of either NADP+ or NADPH: the NADPH/NADP+ remain permanently bound to domain III and are alternately oxidized by acetylpyridine adenine dinucleotide and then reduced by NADH in domain I	Rhodospirillum rubrum	NADP+ + reduced 3-acetylpyridine adenine dinucleotide	-	?

Subunits

Subunits	Comment	Organism
?	domain I contains the binding site for NAD+ and NADH, domain III for NADP+ and NADPH	Rhodospirillum rubrum
?	domain I exists as a seperate polypeptide that can be removed from everted membrane vesicle i.e. chromatophores	Rhodospirillum rubrum

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Bos taurus
NAD+	-	Rhodospirillum rubrum
NADH	-	Bos taurus
NADH	-	Rhodospirillum rubrum
NADP+	-	Bos taurus
NADP+	-	Rhodospirillum rubrum
NADPH	-	Bos taurus
NADPH	-	Rhodospirillum rubrum

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Release 2023.1 (January 2023)