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Literature summary for 1.6.1.2 extracted from

  • Diggle, C.; Quirk, P.G.; Bizouarnt, T.; Grimley, R.L.; Cottont, N.P.J.; Thomas, C.M.; Jackson, J.B.
    Mutation of Tyr235 in the NAD(H)-binding subunit of the proton-translocating nicotinamide nucleotide transhydrogenase of Rhodospirillum rubrum affects the conformational dynamics of a mobile loop and lowers the catalytic activity of the enzyme (1996), J. Biol. Chem., 271, 10109-10115.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression of domain I Y235N and Y235F mutants in Escherichia coli Rhodospirillum rubrum

Protein Variants

Protein Variants Comment Organism
Y235F mutation resides in the soluble NAD(H)-binding peripheral membrane subunit, i.e. domain I, reconstitution of depleted membranes with mutant domain I gives 44% of activity that is obtained with wild-type domain I reconstituted membranes Rhodospirillum rubrum
Y235N mutation resides in the soluble NAD(H)-binding peripheral membrane subunit, i.e. domain I, reconstitution of depleted membranes with mutant domain I gives 18% of activity that is obtained with wild-type domain I reconstituted membranes Rhodospirillum rubrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 NADPH Y235N and Y235F mutants of domain I Rhodospirillum rubrum
0.03
-
 NADPH wild-type domain I Rhodospirillum rubrum
0.06
-
 oxidized 3-acetylpyridine adenine dinucleotide wild-type domain I Rhodospirillum rubrum
0.6
-
 oxidized 3-acetylpyridine adenine dinucleotide Y235F mutant of domain I Rhodospirillum rubrum
0.8
-
 oxidized 3-acetylpyridine adenine dinucleotide Y235N mutant of domain I Rhodospirillum rubrum

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + oxidized 3-acetylpyridine adenine dinucleotide
-
 Rhodospirillum rubrum NADP+ + reduced 3-acetylpyridine adenine dinucleotide
-
 r

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Rhodospirillum rubrum
NADH
-
 Rhodospirillum rubrum
NADP+
-
 Rhodospirillum rubrum
NADPH
-
 Rhodospirillum rubrum