Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.6.1.2 extracted from

  • Jacobs, E.; Heriot, K.; Fisher, R.R.
    Resolution and reconstitution of Rhodospirillum rubrum pyridine dinucleotide transhydrogenase. II. Solubilization of the membrane-bound component (1977), Arch. Microbiol., 115, 151-156.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
lysolecithin aprrox. 0.3%, 150% activation of activity in chromatophore extracts Rhodospirillum rubrum

General Stability

General Stability Organism
solubilized membrane component is unstable loosing all activity when stored overnight at 4°C or -70°C, stable at -70°C in the presence of 0.025 mM NADP+ for several months, inactivation by refreezing after thawing Rhodospirillum rubrum
trypsinolysis is stimulated several fold by NADPH and NADP+, half-maximal stimulation with 0.001-0.002 mM NADPH and 0.002-0.003 mM NADP+, Mg2+ protects from NADP+ stimulated inactivation Rhodospirillum rubrum

Localization

Localization Comment Organism GeneOntology No. Textmining
chromatophore enzyme is compromised of a membrane-bound component and an easily dissociable soluble factor Rhodospirillum rubrum
-
-

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Storage Stability

Storage Stability Organism
-70°C or 4°C, Rhodospirillum rubrum membrane component, inactivation in 1 day, stabilization by NADP+ Rhodospirillum rubrum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44
-
2 min, solubilized membrane component, 50% inactivation Rhodospirillum rubrum
48
-
2 min, membrane particles, 50% inactivation Rhodospirillum rubrum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rhodospirillum rubrum
NADH
-
Rhodospirillum rubrum
NADP+
-
Rhodospirillum rubrum
NADPH
-
Rhodospirillum rubrum