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Literature summary for 1.6.1.2 extracted from
- The kinetic mechanism of pyridine nucleotide transhydrogenase from Escherichia coli (1979), J. Biol. Chem., 254, 888-893.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-AMP | - |
Escherichia coli |  |
| 5'-AMP | competitive vs acetylpyridine adenine dinucleotide, noncompetitive vs. NADPH | Escherichia coli | |
| acetylpyridine adenine dinucleotide | - |
Escherichia coli | |
| NADP+ | - |
Escherichia coli | |
| NADPH | - |
Escherichia coli | |
| reduced acetylpyridine adenine dinucleotide | competitive vs. oxidized acetylpyridine dinucleotide, noncompetitive vs. NADPH | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
NADPH | - |
Escherichia coli | |
| 0.028 | - |
acetylpyridine adenine dinucleotide | - |
Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADPH + 3-acetylpyridine-NAD+ | - |
Escherichia coli | 3-acetylpyridine-NADH + NADP+ | - |
? | |
| NADPH + oxidized 3-acetylpyridine adenine dinucleotide | - |
Escherichia coli | NADP+ + reduced 3-acetylpyridine adenine dinucleotide | - |
r | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Escherichia coli | |
| NADH | - |
Escherichia coli | |
| NADP+ | - |
Escherichia coli | |
| NADPH | - |
Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0035 | - |
NADPH | - |
Escherichia coli | |
| 0.007 | - |
acetylpyridine adenine dinucleotide | - |
Escherichia coli | |
| 0.093 | - |
reduced acetylpyridine adenine dinucleotide | vs. NADPH | Escherichia coli | |
| 0.12 | - |
reduced acetylpyridine adenine dinucleotide | vs. oxidized acetylpyridine adenine dinucleotide | Escherichia coli | |
| 0.17 | - |
NADP+ | vs. NADPH | Escherichia coli | |
| 0.26 | - |
NADPH | vs. oxidized acetylpyridine adenine dinucleotide | Escherichia coli | |
| 2.3 | - |
2'-AMP | vs. NADPH | Escherichia coli | |
| 3.4 | - |
5'-AMP | vs. oxidized acetylpyridine adenine dinucleotide | Escherichia coli | |
| 3.7 | - |
5'-AMP | vs. NADPH | Escherichia coli | |
| 8 | - |
2'-AMP | vs. oxidized acetylpyridine adenine dinucleotide | Escherichia coli | |
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