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Literature summary for 1.5.99.6 extracted from
- Microbial spermidine dehydrogenase: Purification and properties of the enzyme in Pseudomonas aeruginosa and Citrobacter freundii (1990), J. Ferment. Bioeng., 69, 335-340.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Citrobacter freundii |  |
| Hg2+ | - |
Pseudomonas aeruginosa | |
| HgCl2 | inhibition almost complete | Citrobacter freundii | |
| HgCl2 | inhibition by 30% | Pseudomonas aeruginosa | |
| p-chloromercuribenzoate | slight inhibition | Citrobacter freundii | |
| p-chloromercuribenzoate | slight inhibition | Pseudomonas aeruginosa | |
| p-chloromercuribenzoate | - |
Serratia marcescens | |
| Quinacrine | - |
Citrobacter freundii | |
| Quinacrine | - |
Serratia marcescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0104 | - |
spermine | enzyme I | Citrobacter freundii | |
| 0.0143 | - |
spermine | enzyme I | Citrobacter freundii | |
| 0.0183 | - |
spermidine | enzyme II | Citrobacter freundii | |
| 0.0244 | - |
spermidine | enzyme I | Citrobacter freundii | |
| 0.037 | - |
spermidine | - |
Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Citrobacter freundii |
| 43000 | - |
gel filtration | Pseudomonas aeruginosa |
| 63000 | - |
SDS-PAGE | Citrobacter freundii |
| 63000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
| 76000 | - |
- |
Serratia marcescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
IFO12681 | - |
| Citrobacter freundii IFO12681 | - |
IFO12681 | - |
| Flavobacterium dormitator | - |
var. | - |
| Pseudomonas aeruginosa | - |
IFO3080 | - |
| Pseudomonas aeruginosa IFO3080 | - |
IFO3080 | - |
| Serratia marcescens | - |
IFO3046 | - |
| Serratia marcescens IFO3046 | - |
IFO3046 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Citrobacter freundii |
- |
Pseudomonas aeruginosa |
- |
Serratia marcescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.52 | - |
- |
Pseudomonas aeruginosa |
| 97.5 | - |
- |
Citrobacter freundii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| spermidine + 2,6-dichlorophenolindophenol | - |
Pseudomonas aeruginosa | 1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol | - |
? | |
| spermidine + 2,6-dichlorophenolindophenol | - |
Serratia marcescens | 1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol | - |
? | |
| spermidine + 2,6-dichlorophenolindophenol | - |
Citrobacter freundii | 1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol | - |
? | |
| spermidine + 2,6-dichlorophenolindophenol | - |
Citrobacter freundii IFO12681 | 1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol | - |
? | |
| spermidine + 2,6-dichlorophenolindophenol | - |
Pseudomonas aeruginosa IFO3080 | 1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol | - |
? | |
| spermidine + 2,6-dichlorophenolindophenol | - |
Serratia marcescens IFO3046 | 1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol | - |
? | |
| spermidine + electron acceptor | - |
Pseudomonas aeruginosa | 1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor | - |
? | |
| spermidine + electron acceptor | - |
Serratia marcescens | 1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor | - |
? | |
| spermidine + electron acceptor | - |
Citrobacter freundii | 1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor | - |
? | |
| spermidine + electron acceptor | - |
Flavobacterium dormitator | 1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor | - |
? | |
| spermidine + electron acceptor | - |
Citrobacter freundii IFO12681 | 1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor | - |
? | |
| spermidine + electron acceptor | - |
Pseudomonas aeruginosa IFO3080 | 1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor | - |
? | |
| spermidine + electron acceptor | - |
Serratia marcescens IFO3046 | 1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor | - |
? | |
| spermidine + K3Fe(CN)6 | - |
Pseudomonas aeruginosa | 1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide | - |
? | |
| spermidine + K3Fe(CN)6 | - |
Serratia marcescens | 1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide | - |
? | |
| spermidine + K3Fe(CN)6 | - |
Citrobacter freundii | 1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide | - |
? | |
| spermidine + K3Fe(CN)6 | - |
Citrobacter freundii IFO12681 | 1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide | - |
? | |
| spermidine + K3Fe(CN)6 | - |
Pseudomonas aeruginosa IFO3080 | 1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide | - |
? | |
| spermidine + K3Fe(CN)6 | - |
Serratia marcescens IFO3046 | 1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide | - |
? | |
| spermine + electron acceptor | - |
Pseudomonas aeruginosa | ? + reduced electron acceptor | - |
? | |
| spermine + electron acceptor | - |
Serratia marcescens | ? + reduced electron acceptor | - |
? | |
| spermine + electron acceptor | - |
Citrobacter freundii | ? + reduced electron acceptor | - |
? | |
| spermine + electron acceptor | - |
Citrobacter freundii IFO12681 | ? + reduced electron acceptor | - |
? | |
| spermine + electron acceptor | - |
Pseudomonas aeruginosa IFO3080 | ? + reduced electron acceptor | - |
? | |
| spermine + electron acceptor | - |
Serratia marcescens IFO3046 | ? + reduced electron acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Serratia marcescens |
| monomer | 1 * 63000, SDS-PAGE | Pseudomonas aeruginosa |
| monomer | 1 * 63000, SDS-PAGE | Citrobacter freundii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | - |
Pseudomonas aeruginosa |
| 50 | - |
- |
Citrobacter freundii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable below | Pseudomonas aeruginosa |
| 50 | - |
stable below | Citrobacter freundii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Citrobacter freundii |
| 7.2 | - |
- |
Serratia marcescens |
| 8 | - |
- |
Pseudomonas aeruginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | - |
Pseudomonas aeruginosa |
| 7 | 8 | - |
Citrobacter freundii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Citrobacter freundii | |
| heme | - |
Serratia marcescens | |
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