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Literature summary for 1.5.99.6 extracted from

  • Tabor, C.W.; Kellog, P.D.
    Spermidine dehydrogenase (Serratia marcescens) (1971), Methods Enzymol., 17B, 746-753.
No PubMed abstract available
Search for more literature for 1.5.99.6

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate
-
 Serratia marcescens
phenylhydrazine
-
 Serratia marcescens
Quinacrine
-
 Serratia marcescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information at optimal pH Km for spermidine less than 0.0001 mM Serratia marcescens
0.05
-
 spermine
-
 Serratia marcescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
-
 Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
420
-
-
 Serratia marcescens

Storage Stability

Storage Stability Organism
-20°C, can be stored in supernatant solution for several weeks without loss of activity Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3'-diaminodipropylamine + electron acceptor
-
 Serratia marcescens ? + reduced electron acceptor
-
 ?
monoacetylspermidine + electron acceptor
-
 Serratia marcescens ? + reduced electron acceptor
-
 ?
N,N'-di(aminopropyl)-propanediamine + electron acceptor
-
 Serratia marcescens ? + reduced electron acceptor
-
 ?
spermidine + 2,6-dichlorophenolindophenol
-
 Serratia marcescens 1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
 ?
spermidine + electron acceptor
-
 Serratia marcescens 1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
 ?
spermidine + K3Fe(CN)6
-
 Serratia marcescens 1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
 ?
spermidine + phenazine methosulfate
-
 Serratia marcescens 1,3-diaminopropane + DELTA1-pyrroline + reduced phenazine methosulfate
-
 ?
spermine + electron acceptor
-
 Serratia marcescens ? + reduced electron acceptor
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 spermidine as substrate Serratia marcescens
8.8
-
 spermine as substrate Serratia marcescens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Serratia marcescens
iron-protoporphyrin IX
-
 Serratia marcescens