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Literature summary for 1.5.99.6 extracted from

  • Tabor, C.W.; Kellog, P.D.
    Identification of flavin adenine dinucleotide and heme in a homogeneous spermidine dehydrogenase from Serratia marcescens (1970), J. Biol. Chem., 245, 5424-5433.
    View publication on PubMed
Search for more literature for 1.5.99.6

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 sedimentation equilibrium centrifugation, centrifugation in a sucrose density gradient Serratia marcescens
79000
-
 sedimentation velocity centrifugation, diffusion coefficient Serratia marcescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
spermidine + cytochrome c Serratia marcescens natural electron acceptor horse heart cytochrome c 1,3-diaminopropane + gamma-aminobutylaldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
 ATCC 25179
-

Purification (Commentary)

Purification (Comment) Organism
-
 Serratia marcescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
420
-
-
 Serratia marcescens

Storage Stability

Storage Stability Organism
-20°C, can be stored in 0.1 M potassium phosphate buffer, pH 7.2 for over 6 months with less than 10% loss of activity Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
monoacetylspermidine + electron acceptor
-
 Serratia marcescens ? + reduced electron acceptor
-
 ?
N,N'-bis(3-aminopropyl)-1,3-propanediamine + electron acceptor
-
 Serratia marcescens ? + reduced electron acceptor
-
 ?
N-(3-aminopropyl)-1,3-propanediamine + electron acceptor
-
 Serratia marcescens ? + reduced electron acceptor
-
 ?
N-(3-hydroxypropyl)-1,4-diaminobutane + electron acceptor
-
 Serratia marcescens ? + reduced electron acceptor
-
 ?
spermidine + 2,6-dichlorophenolindophenol
-
 Serratia marcescens 1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
 ?
spermidine + cytochrome c natural electron acceptor horse heart cytochrome c Serratia marcescens 1,3-diaminopropane + gamma-aminobutylaldehyde
-
 ?
spermidine + electron acceptor
-
 Serratia marcescens 1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
 ?
spermidine + K3Fe(CN)6
-
 Serratia marcescens 1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
 ?
spermidine + phenazine methosulfate
-
 Serratia marcescens 1,3-diaminopropane + DELTA1-pyrroline + reduced phenazine methosulfate
-
 ?
spermine + electron acceptor
-
 Serratia marcescens ? + reduced electron acceptor
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 76000, SDS-PAGE Serratia marcescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
 stable when heated for 5 min Serratia marcescens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Serratia marcescens
heme
-
 Serratia marcescens
iron-protoporphyrin IX
-
 Serratia marcescens