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Literature summary for 1.5.99.5 extracted from

  • Boulton, C.A.; Haywood, G.W.; Large, P.J.
    N-Methylglutamate dehydrogenase, a flavohaemoprotein purified from a new pink trimethylamine utilizing bacterium (1980), J. Gen. Microbiol., 117, 293-304.
No PubMed abstract available
Search for more literature for 1.5.99.5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 2,6-dichlorophenolindophenol
-
 bacterium AT2
0.333
-
 N-Methyl-L-glutamate
-
 bacterium AT2

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 bacterium AT2 5829
-
membrane
-
 Pseudomonas sp. 16020
-
membrane
-
 Aminobacter aminovorans 16020
-
membrane
-
 bacterium AT2 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
108000
-
 4 * 108000 bacterium AT2
407000
-
 gel filtration bacterium AT2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-methyl-L-glutamate + acceptor + H2O Pseudomonas sp.
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O Aminobacter aminovorans
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O Pseudomonas methylica
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O Hyphomicrobium vulgare
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O bacterium AT2
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O Hyphomicrobium vulgare ZV
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O Hyphomicrobium vulgare 3
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Aminobacter aminovorans
-
-
-
Aminobacter aminovorans AM1
-
-
-
bacterium AT2
-
 pink facultative methylotroph
-
Hyphomicrobium vulgare
-
-
-
Hyphomicrobium vulgare 3
-
-
-
Hyphomicrobium vulgare ZV
-
-
-
Pseudomonas methylica
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. MA / M.A. / ATCC 23819
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 bacterium AT2
partial Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.009
-
 specific activity after growth on trimethylamine bacterium AT2
0.0161
-
-
 Hyphomicrobium vulgare
0.02
-
-
 Aminobacter aminovorans
0.027
-
 specific activity after growth on methylamine bacterium AT2
0.0437
-
 purified enzyme bacterium AT2
0.113
-
-
 bacterium AT2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
 Pseudomonas sp. ?
-
 ?
additional information
-
 Aminobacter aminovorans ?
-
 ?
additional information not active with NAD+, ferricyanide cytochrome c or horse heart cytochrome c, no activity detected with N-methyl-D-alanine bacterium AT2 ?
-
 ?
additional information
-
 Aminobacter aminovorans AM1 ?
-
 ?
additional information
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 ?
-
 ?
N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
-
 bacterium AT2 DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
 bacterium AT2 DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans AM1 DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
 bacterium AT2 L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans AM1 L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl-DL-glutamate + 2,6-dichlorophenolindophenol + H2O
-
 bacterium AT2 DL-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl-L-glutamate + acceptor + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O
-
 Aminobacter aminovorans L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O
-
 Pseudomonas methylica L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O
-
 Hyphomicrobium vulgare L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O
-
 bacterium AT2 L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O
-
 Hyphomicrobium vulgare ZV L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O
-
 Hyphomicrobium vulgare 3 L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methylglutamate + N,N,N,N-tetramethyl-o-phenylene diamine + H2O
-
 bacterium AT2 L-glutamate + formaldehyde + reduced N,N,N,N-tetramethyl-o-phenylene diamine
-
 ?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 bacterium AT2 L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans AM1 L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methylglutamate + phenazine methosulfate + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
N-methylglutamate + phenazine methosulfate + H2O
-
 Aminobacter aminovorans L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
N-methylglutamate + phenazine methosulfate + H2O
-
 bacterium AT2 L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
N-methylglutamate + phenazine methosulfate + H2O
-
 Aminobacter aminovorans AM1 L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
N-methylglutamate + phenazine methosulfate + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
N-methylglutamate + radical cation of 2,2'-azinodi-[3-ethylbenzthiazoline 6-sulfonate] + H2O
-
 bacterium AT2 L-glutamate + formaldehyde + reduced acceptor
-
 ?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
 bacterium AT2 aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Aminobacter aminovorans
tetramer 4 * 108000 bacterium AT2

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 half-life of the purified enzyme 35 min at 40°C, 10.5 min at 45°C and 1 min at 50°C bacterium AT2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 bacterium AT2

Cofactor

Cofactor Comment Organism Structure
FAD
-
 bacterium AT2
NAD+
-
 Pseudomonas methylica
NAD+
-
 Hyphomicrobium vulgare