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Literature summary for 1.5.99.5 extracted from
- Solubilization, partial purification and properties of N-methylglutamate dehydrogenase from Pseudomonas aminovorans (1977), Biochem. J., 161, 357-370.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Aminobacter aminovorans |  |
| Cu2+ | total inhibition at 1 mM | Aminobacter aminovorans | |
| formaldehyde | - |
Aminobacter aminovorans | |
| iodoacetamide | - |
Aminobacter aminovorans | |
| p-chloromercuribenzoate | - |
Aminobacter aminovorans | |
| Triton X-100 | - |
Aminobacter aminovorans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
N-Methyl-L-glutamate | - |
Aminobacter aminovorans | |
| 0.21 | - |
2,6-dichlorophenolindophenol | - |
Aminobacter aminovorans | |
| 0.46 | - |
N-methyl DL-aspartate | - |
Aminobacter aminovorans | |
| 25 | - |
N-methyl L-alanine | - |
Aminobacter aminovorans | |
| 33 | - |
N-methyl D-alanine | - |
Aminobacter aminovorans | |
| 87 | - |
N-methyl DL-alanine | - |
Aminobacter aminovorans | |
| 90.9 | - |
sarcosine | - |
Aminobacter aminovorans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Aminobacter aminovorans | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM stimulates activity by 33% | Aminobacter aminovorans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-methyl-L-glutamate + acceptor + H2O | Aminobacter aminovorans | - |
L-glutamate + formaldehyde + reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aminobacter aminovorans | - |
- |
- |
| Aminobacter aminovorans AM1 | - |
- |
- |
| Aminobacter aminovorans N.C.I.B. 9039 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Aminobacter aminovorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00033 | - |
crude extract, grown on trimethylamine N-oxide | Aminobacter aminovorans |
| 0.0018 | - |
crude extract, grown on trimethylamine | Aminobacter aminovorans |
| 0.0025 | - |
crude extract, grown on methylamine | Aminobacter aminovorans |
| 0.00364 | - |
crude extract, grown on dimethylamine | Aminobacter aminovorans |
| 0.0554 | - |
- |
Aminobacter aminovorans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, stable to repeated freezing and thawing and storage at a protein concentration of 3 mg/ml | Aminobacter aminovorans |
| -196°C, survives cooling with subsequent thawing | Aminobacter aminovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-methyl D-alanine + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans | D-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans | DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans | DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans AM1 | DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans N.C.I.B. 9039 | DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans | L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans AM1 | L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans N.C.I.B. 9039 | L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans | L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans AM1 | L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans N.C.I.B. 9039 | L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methyl-L-glutamate + acceptor + H2O | - |
Aminobacter aminovorans | L-glutamate + formaldehyde + reduced acceptor | - |
? | |
| N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans | L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans AM1 | L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans N.C.I.B. 9039 | L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N-methylglutamate + phenazine methosulfate + H2O | - |
Aminobacter aminovorans | L-glutamate + formaldehyde + reduced phenazine methosulfate | - |
? | |
| N-methylglutamate + phenazine methosulfate + H2O | - |
Aminobacter aminovorans AM1 | L-glutamate + formaldehyde + reduced phenazine methosulfate | - |
? | |
| N-methylglutamate + phenazine methosulfate + H2O | - |
Aminobacter aminovorans N.C.I.B. 9039 | L-glutamate + formaldehyde + reduced phenazine methosulfate | - |
? | |
| sarcosine + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans | aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | heat-labile with half-lives of 35 min at 30°C and 5 min at 40°C | Aminobacter aminovorans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | - |
Aminobacter aminovorans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
acid labile, stable at neutrality and pH values above 7.0 | Aminobacter aminovorans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b | - |
Aminobacter aminovorans |
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