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Literature summary for 1.5.99.4 extracted from

  • Hochstein, L.L.; Dalton, B.P.
    The purification and properties of nicotine oxidase (1967), Biochim. Biophys. Acta, 139, 56-68.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 47% inhibition at 2 mM Pseudarthrobacter oxydans
2,2'-dipyridyl 27% inhibition at 2 mM Pseudarthrobacter oxydans
Acriflavine 64% inhibition at 1 mM Pseudarthrobacter oxydans
brilliant cresyl blue concentration over 0.2 mM Pseudarthrobacter oxydans
Cu2+
-
Pseudarthrobacter oxydans
cyanide 95% inhibition at 1 mM potassium cyanide Pseudarthrobacter oxydans
Hg2+ 96% inhibition at 0.1 mM Pseudarthrobacter oxydans
iodoacetamide 23% inhibition at 10 mM Pseudarthrobacter oxydans
additional information riboflavin 5'-phosphate protects enzyme against acriflavine inhibition, FMN protects enzyme against acriflavine inhibition, FAD only partially effective, glutathione protects against p-chloromercuriphenylsulfonic inhibition Pseudarthrobacter oxydans
p-chloromercuriphenylsulfonic acid 62% inhibition at 1 mM Pseudarthrobacter oxydans
Quinacrine 49% inhibition at 0.1 mM Pseudarthrobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
brilliant cresyl blue
-
Pseudarthrobacter oxydans
0.027
-
methylene blue
-
Pseudarthrobacter oxydans
0.037
-
Nicotine
-
Pseudarthrobacter oxydans
0.49
-
menadione
-
Pseudarthrobacter oxydans
1
-
vitamin K5
-
Pseudarthrobacter oxydans
1.24
-
2,6-dichlorophenolindophenol
-
Pseudarthrobacter oxydans

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ inhibitory Pseudarthrobacter oxydans

Organism

Organism UniProt Comment Textmining
Pseudarthrobacter oxydans
-
P-34 DSM 419
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, heat denaturation, TEAE-cellulose chromatography Pseudarthrobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29.2
-
-
Pseudarthrobacter oxydans

Storage Stability

Storage Stability Organism
-19°C, 1 month, without thawing and refreezing, without loss of activity Pseudarthrobacter oxydans
-19°C, cells, 4 months, without loss of activity Pseudarthrobacter oxydans
4°C, 2-3 days, no significant loss of activity Pseudarthrobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O
-
Pseudarthrobacter oxydans (S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
r
(S)-nicotine + brilliant cresyl blue + H2O
-
Pseudarthrobacter oxydans (S)-6-hydroxynicotine + reduced brilliant cresyl blue
-
r
(S)-nicotine + menadione + H2O
-
Pseudarthrobacter oxydans (S)-6-hydroxynicotine + menadiol
-
r
(S)-nicotine + methylene blue + H2O
-
Pseudarthrobacter oxydans (S)-6-hydroxynicotine + reduced methylene blue
-
r
(S)-nicotine + vitamin K5 + H2O
-
Pseudarthrobacter oxydans (S)-6-hydroxynicotine + reduced vitamin K5
-
r
anabasine + acceptor + H2O
-
Pseudarthrobacter oxydans 6-hydroxyanabasine + reduced acceptor
-
?
myosmine + acceptor + H2O
-
Pseudarthrobacter oxydans ? + reduced acceptor
-
?
nicotine + acceptor + H2O brilliant cresyl blue, methylene blue, 5-hydroxy-1,4-naphthoquinone, 2,6-dichlorophenolindophenol, menadione and vitamin K5 used as electron acceptors Pseudarthrobacter oxydans 6-hydroxynicotine + reduced acceptor
-
?
nicotine-N-oxide + acceptor + H2O
-
Pseudarthrobacter oxydans 6-hydroxynicotine-N-oxide + reduced acceptor
-
?
nicotinic acid + acceptor + H2O
-
Pseudarthrobacter oxydans 6-hydroxynicotinic acid + reduced acceptor
-
?
nornicotine + acceptor + H2O
-
Pseudarthrobacter oxydans 6-hydroxynornicotine + reduced acceptor
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudarthrobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 8.2 sodium diphosphate buffer Pseudarthrobacter oxydans
7.9
-
assay at Pseudarthrobacter oxydans
8.6 9 Tris chloride or Tris phosphate buffer Pseudarthrobacter oxydans

Cofactor

Cofactor Comment Organism Structure
FAD partially protects the enzyme Pseudarthrobacter oxydans
FMN metalloflavoprotein, protects enzyme against inhibition by acriflavine Pseudarthrobacter oxydans
additional information metalloflavoprotein Pseudarthrobacter oxydans