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Literature summary for 1.5.99.13 extracted from

  • Tani, Y.; Tanaka, K.; Yabutani, T.; Mishima, Y.; Sakuraba, H.; Ohshima, T.; Motonaka, J.
    Development of a D-amino acids electrochemical sensor based on immobilization of thermostable D-proline dehydrogenase within agar gel membrane (2008), Anal. Chim. Acta, 619, 215-220.
    View publication on PubMed
Search for more literature for 1.5.99.13

Application

Application Comment Organism
analysis development of a D-amino acids electrochemical sensor based on immobilization of thermostable D-proline dehydrogenase within agar gel membrane. The D-Pro DH/agar immobilized electrode is applied to determination of D-amino acids in a human urine sample Pyrobaculum islandicum

General Stability

General Stability Organism
enzyme activity in the D-Pro dehydrogenase/agar immobilized electrode is retained over 90% for 80 days Pyrobaculum islandicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.9
-
 D-proline
-
 Pyrobaculum islandicum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated to Pyrobaculum islandicum 16020
-

Organism

Organism UniProt Comment Textmining
Pyrobaculum islandicum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + oxidized 2,6-dichloroindophenol + H2O 9% of the activity with D-proline Pyrobaculum islandicum pyruvate + NH3 + reduced 2,6-dichloroindophenol
-
 ?
D-aspartate + oxidized 2,6-dichloroindophenol + H2O 7% of the activity with D-proline Pyrobaculum islandicum oxaloacetate + NH3 + reduced 2,6-dichloroindophenol
-
 ?
D-histidine + oxidized 2,6-dichloroindophenol + H2O 29% of the activity with D-proline Pyrobaculum islandicum 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol
-
 ?
D-isoleucine + oxidized 2,6-dichloroindophenol + H2O 46% of the activity with D-proline Pyrobaculum islandicum 3-methyl-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol
-
 ?
D-leucine + oxidized 2,6-dichloroindophenol + H2O 19% of the activity with D-proline Pyrobaculum islandicum 4-methyl-2-oxopentanoate + NH3 + reduced 2,6-dichloroindophenol
-
 ?
D-proline + oxidized 2,6-dichloroindophenol
-
 Pyrobaculum islandicum DELTA1-pyrroline 2-carboxylate + reduced 2,6-dichloroindophenol
-
 ?
D-valine + oxidized 2,6-dichloroindophenol + H2O 18% of the activity with D-proline Pyrobaculum islandicum 2-oxoisopentanoate + NH3 + reduced 2,6-dichloroindophenol
-
 ?

Synonyms

Synonyms Comment Organism
D-Pro DH
-
 Pyrobaculum islandicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Pyrobaculum islandicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pyrobaculum islandicum

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Pyrobaculum islandicum