Cloned (Comment) | Organism |
---|---|
expressed as extracellular protein in Saccharomyces cerevisae | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
N6-(2-isopentenyl)adenine | with 2,3-dimethoxy-5-methyl-p-benzoquinone, pH 7.0, 37°C | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53300 | - |
x * 61000, SDS-PAGE, weak bands also at 75000 Da and100000 Da, x * 53300, calculated from the deduced amino acid sequence | Arabidopsis thaliana |
61000 | - |
x * 61000, SDS-PAGE, weak bands also at 75000 Da and100000 Da, x * 53300, calculated from the deduced amino acid sequence | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | three N-glycosylation sites, glycosylation of the enzyme can explain the difference between theoretical and experimental weight | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
recombinant protein from culture medium | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activities given for all possible reactant pairs | Arabidopsis thaliana |
0.0052 | - |
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-p-benzoquinone, pH 7.0, 37°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kinetin + 2,3-dimethoxy-5-methyl-p-benzoquinone | low activity | Arabidopsis thaliana | ? | - |
? | |
m-topolin + 2,3-dimethoxy-5-methyl-p-benzoquinone | very low activity | Arabidopsis thaliana | ? | - |
? | |
additional information | irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde | Arabidopsis thaliana | ? | - |
? | |
additional information | AtCKX2 prefers p-quinones and 2,6-dichlorophenol indophenol as electron acceptors, low reactivity with oxygen | Arabidopsis thaliana | ? | - |
? | |
additional information | no activity with dihydrozeatin | Arabidopsis thaliana | ? | - |
? | |
additional information | no activity with vitamin K1 as electron acceptor | Arabidopsis thaliana | ? | - |
? | |
N6-(2-isopentenyl)adenine + 1,4-naphthoquinone | very effective electron acceptor | Arabidopsis thaliana | ? | - |
? | |
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-p-benzoquinone | - |
Arabidopsis thaliana | ? | - |
? | |
N6-(2-isopentenyl)adenine + 2,6-dichlorophenol indophenol | very effective electron acceptor | Arabidopsis thaliana | ? | - |
? | |
N6-(2-isopentenyl)adenine + CuCl2 | low activity | Arabidopsis thaliana | ? | - |
? | |
N6-(2-isopentenyl)adenine + duroquinone | low activity | Arabidopsis thaliana | ? | - |
? | |
N6-(2-isopentenyl)adenine + O2 | low activity | Arabidopsis thaliana | ? | - |
? | |
N6-(2-isopentenyl)adenine + potassium ferricyanide | low activity | Arabidopsis thaliana | ? | - |
? | |
N6-(2-isopentenyl)adenine + vitamin K3 | low activity | Arabidopsis thaliana | ? | - |
? | |
N6-(2-isopentenyl)adenine 9-glucoside + 2,3-dimethoxy-5-methyl-p-benzoquinone | low activity | Arabidopsis thaliana | ? | - |
? | |
N6-(2-isopentenyl)adenine 9-riboside + 2,3-dimethoxy-5-methyl-p-benzoquinone | - |
Arabidopsis thaliana | ? | - |
? | |
N6-(2-isopentenyl)adenine 9-riboside-5'-monophosphate + 2,3-dimethoxy-5-methyl-p-benzoquinone | low activity | Arabidopsis thaliana | ? | - |
? | |
N6-methyl-isopentenyladenine + 2,3-dimethoxy-5-methyl-p-benzoquinone | very low activity | Arabidopsis thaliana | ? | - |
? | |
o-topolin + 2,3-dimethoxy-5-methyl-p-benzoquinone | very low activity | Arabidopsis thaliana | ? | - |
? | |
p-topolin + 2,3-dimethoxy-5-methyl-p-benzoquinone | very low activity | Arabidopsis thaliana | ? | - |
? | |
trans-zeatin + 2,3-dimethoxy-5-methyl-p-benzoquinone | very effective substrate | Arabidopsis thaliana | ? | - |
? | |
trans-zeatin 9-glucoside + 2,3-dimethoxy-5-methyl-p-benzoquinone | low activity | Arabidopsis thaliana | ? | - |
? | |
trans-zeatin 9-riboside + 2,3-dimethoxy-5-methyl-p-benzoquinone | very effective substrate | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | x * 61000, SDS-PAGE, weak bands also at 75000 Da and100000 Da, x * 53300, calculated from the deduced amino acid sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtCKX2 | seven distinct genes AtCKX1 to AtCKX7, AtCKX2 is subject of the study | Arabidopsis thaliana |
CKX | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.4 | - |
N6-(2-isopentenyl)adenine | with 2,3-dimethoxy-5-methyl-p-benzoquinone, pH 7.0, 37°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | with 2,3-dimethoxy-5-methyl-p-benzoquinone | Arabidopsis thaliana |
8 | - |
with 2,6-dichlorophenol indophenol | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arabidopsis thaliana |