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Literature summary for 1.5.99.12 extracted from

  • Kopecny, D.; Pethe, C.; Sebela, M.; Houba-Herin, N.; Madzak, C.; Majira, A.; Laloue, M.
    High-level expression and characterization of Zea mays cytokinin oxidase/dehydrogenase in Yarrowia lipolytica (2005), Biochimie, 87, 1011-1022.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
high-level expression of cytokinin oxidase/dehydrogenase in Yarrowia lipolytica Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
N6-isopentenyladenine 30°C, pH 7.4 Zea mays
0.009
-
zeatin 30°C, pH 7.4 Zea mays
0.012
-
N6-isopentenyladenosine 30°C, pH 7.4 Zea mays
0.063
-
zeatin riboside 30°C, pH 7.4 Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5600
-
1 * 5600, deglycosylated enzyme, SDS-PAGE Zea mays
64000
-
gel filtration, MALDI-MS Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays Q9T0N8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 22% neutral sugars Zea mays

Purification (Commentary)

Purification (Comment) Organism
-
Zea mays

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
62.4
-
-
Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N6-isopentenyladenine + 2,6-dichlorophenolindophenol
-
Zea mays adenine + 3-methyl-2-butenal + reduced 2,6-dichlorophenolindophenol
-
?
N6-isopentenyladenosine + 2,6-dichlorophenolindophenol
-
Zea mays 3-methylbut-2-enal + adenosine + reduced 2,6-dichlorophenolindophenol
-
?
zeatin + 2,6-dichlorophenolindophenol
-
Zea mays 4-hydroxy-3-methylbut-2-enal + adenine + reduced 2,6-dichlorophenolindophenol
-
?
zeatin riboside + 2,6-dichlorophenolindophenol
-
Zea mays ? + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

Subunits Comment Organism
monomer 1 * 5600, deglycosylated enzyme, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
cytokinin oxidase/dehydrogenase
-
Zea mays
ZmCKO1
-
Zea mays

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
T50 for 30 min incubation Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21
-
zeatin riboside 30°C, pH 7.4 Zea mays
62
-
N6-isopentenyladenosine 30°C, pH 7.4 Zea mays
128
-
N6-isopentenyladenine 30°C, pH 7.4 Zea mays
135
-
zeatin 30°C, pH 7.4 Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
reaction withz zeatin derivatives Zea mays
7.2
-
reaction with N6-(2-isopentenyl)adenine derivatives Zea mays

Cofactor

Cofactor Comment Organism Structure
FAD
-
Zea mays

pI Value

Organism Comment pI Value Maximum pI Value
Zea mays calculated from sequence
-
5.9
Zea mays chromatofocusing
-
6.25