Cloned (Comment) | Organism |
---|---|
high-level expression of cytokinin oxidase/dehydrogenase in Yarrowia lipolytica | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
N6-isopentenyladenine | 30°C, pH 7.4 | Zea mays | |
0.009 | - |
zeatin | 30°C, pH 7.4 | Zea mays | |
0.012 | - |
N6-isopentenyladenosine | 30°C, pH 7.4 | Zea mays | |
0.063 | - |
zeatin riboside | 30°C, pH 7.4 | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
5600 | - |
1 * 5600, deglycosylated enzyme, SDS-PAGE | Zea mays |
64000 | - |
gel filtration, MALDI-MS | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | Q9T0N8 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 22% neutral sugars | Zea mays |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62.4 | - |
- |
Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-isopentenyladenine + 2,6-dichlorophenolindophenol | - |
Zea mays | adenine + 3-methyl-2-butenal + reduced 2,6-dichlorophenolindophenol | - |
? | |
N6-isopentenyladenosine + 2,6-dichlorophenolindophenol | - |
Zea mays | 3-methylbut-2-enal + adenosine + reduced 2,6-dichlorophenolindophenol | - |
? | |
zeatin + 2,6-dichlorophenolindophenol | - |
Zea mays | 4-hydroxy-3-methylbut-2-enal + adenine + reduced 2,6-dichlorophenolindophenol | - |
? | |
zeatin riboside + 2,6-dichlorophenolindophenol | - |
Zea mays | ? + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 5600, deglycosylated enzyme, SDS-PAGE | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
cytokinin oxidase/dehydrogenase | - |
Zea mays |
ZmCKO1 | - |
Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
T50 for 30 min incubation | Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
zeatin riboside | 30°C, pH 7.4 | Zea mays | |
62 | - |
N6-isopentenyladenosine | 30°C, pH 7.4 | Zea mays | |
128 | - |
N6-isopentenyladenine | 30°C, pH 7.4 | Zea mays | |
135 | - |
zeatin | 30°C, pH 7.4 | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reaction withz zeatin derivatives | Zea mays |
7.2 | - |
reaction with N6-(2-isopentenyl)adenine derivatives | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Zea mays |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Zea mays | calculated from sequence | - |
5.9 |
Zea mays | chromatofocusing | - |
6.25 |