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Literature summary for 1.5.8.4 extracted from

  • Yang, T.; Shao, Y.H.; Guo, L.Z.; Meng, X.L.; Yu, H.; Lu, W.D.
    Role of N,N-dimethylglycine and its catabolism to sarcosine in Chromohalobacter salexigens DSM 3043 (2020), Appl. Environ. Microbiol., 86, e01186 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.8.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Chromohalobacter salexigens

Inhibitors

Inhibitors Comment Organism Structure
EDTA 2 mM, 33% inhibition Chromohalobacter salexigens
additional information Na+, K+, Ca2+, Zn2+, Mn2+, Mg2+, Fe2+, and Co2+ display no or slightly inhibitory effects at 2 mM Chromohalobacter salexigens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.6
-
 N,N-dimethylglycine pH 7.5, 60°C Chromohalobacter salexigens
14.65
-
 sarcosine pH 7.5, 60°C Chromohalobacter salexigens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2 mM, 12% increase in activity Chromohalobacter salexigens
additional information Na+, K+, Ca2+, Zn2+, Mn2+, Mg2+, Fe2+, and Co2+ display no or slightly inhibitory effects at 2 mM Chromohalobacter salexigens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79000
-
 gel filtration Chromohalobacter salexigens

Organism

Organism UniProt Comment Textmining
Chromohalobacter salexigens Q1QYW1
-
-
Chromohalobacter salexigens DSM 3043 Q1QYW1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N-dimethylglycine + electron-transfer flavoprotein + H2O
-
 Chromohalobacter salexigens sarcosine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
-
 r
N,N-dimethylglycine + electron-transfer flavoprotein + H2O
-
 Chromohalobacter salexigens DSM 3043 sarcosine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
-
 r
sarcosine + electron-transfer flavoprotein + H2O
-
 Chromohalobacter salexigens glycine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
-
 r
sarcosine + electron-transfer flavoprotein + H2O
-
 Chromohalobacter salexigens DSM 3043 glycine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 77200, SDS-PAGE, 1 * 78507, calculated from sequence Chromohalobacter salexigens

Synonyms

Synonyms Comment Organism
Csal_0990
-
 Chromohalobacter salexigens
DdhC
-
 Chromohalobacter salexigens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Chromohalobacter salexigens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70
-
 64% of maximum activity Chromohalobacter salexigens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
 sarcosine pH 7.5, 60°C Chromohalobacter salexigens
0.32
-
 N,N-dimethylglycine pH 7.5, 60°C Chromohalobacter salexigens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Chromohalobacter salexigens

Cofactor

Cofactor Comment Organism Structure
FAD noncovalently bound flavin adenine dinucleotide, purified protein shows the absorption spectrum of an oxidized flavoprotein, with maxima at 222, 275, 370, and 440 nm and one shoulder at around 470 nm Chromohalobacter salexigens
NAD+ Km value 0.8 mM, NAD+ is preferred over NADP+ Chromohalobacter salexigens
NADP+ Km value 3.2 mM, NAD+ is preferred over NADP+ Chromohalobacter salexigens