Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM | Pseudomonas putida | |
Cu2+ | not in | Pseudomonas putida | |
Hg2+ | 1 mM | Pseudomonas putida | |
additional information | not Co2+, Na+, EDTA, SDS, Triton X-100, semicarbazide; not inhibited by: Ba2+, Ca2+, Fe3+, Li+, Zn2+, dithiothreitol, 2-mercaptoethanol, iodoacetate | Pseudomonas putida | |
PCMB | 2 mM, 100% loss of activity | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.15 | - |
N-Methylalanine | - |
Pseudomonas putida | |
3.9 | - |
sarcosine | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
107000 | - |
SDS-PAGE | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + acceptor + H2O | Pseudomonas putida | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate | glycine + formaldehyde + reduced acceptor | - |
? | |
sarcosine + acceptor + H2O | Pseudomonas putida RS65 | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate | glycine + formaldehyde + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
RS65 | - |
Pseudomonas putida RS65 | - |
RS65 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-methyl-L-alanine + acceptor + H2O | - |
Pseudomonas putida | L-alanine + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-alanine + acceptor + H2O | - |
Pseudomonas putida RS65 | L-alanine + formaldehyde + reduced acceptor | - |
? | |
sarcosine + acceptor + H2O | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate | Pseudomonas putida | glycine + formaldehyde + reduced acceptor | - |
? | |
sarcosine + acceptor + H2O | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate | Pseudomonas putida RS65 | glycine + formaldehyde + reduced acceptor | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | at 25°C for 6 h | Pseudomonas putida |