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Literature summary for 1.5.8.3 extracted from

  • Oka, I.; Yoshimoto, T.; Rikitake, K.; Ogushi, S.; Tsur, D.
    Sarcosine dehydrogenase from Pseudomonas putida: purification and some properties (1979), Agric. Biol. Chem., 43, 1197-1203.
No PubMed abstract available
Search for more literature for 1.5.8.3

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Pseudomonas putida
additional information not inhibited by: Ba2+, Ca2+, Fe3+, Li+, Zn2+, dithiothreitol, 2-mercaptoethanol, iodoacetate; not Mn2+, Fe2+, phenylmethane sulfonylfluoride, dinitrofluorobenzene, tetranitromethane, 5,5`-dithiobis-(2-nitrobenzoate) Pseudomonas putida
PCMB 2 mM, 59% loss of activity Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 phenazine methosulfate
-
 Pseudomonas putida
2
-
 potassium ferricyanide
-
 Pseudomonas putida
11
-
 N-methyl-L-valine
-
 Pseudomonas putida
20
-
 N-Methyl-L-leucine
-
 Pseudomonas putida
29
-
 sarcosine
-
 Pseudomonas putida
69
-
 N-Methylalanine
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42500
-
 4 * 42500, SDS-PAGE Pseudomonas putida
170000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sarcosine + acceptor + H2O Pseudomonas putida acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate glycine + formaldehyde + reduced acceptor
-
 ?
sarcosine + acceptor + H2O Corynebacterium sp. acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate glycine + formaldehyde + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-
Pseudomonas putida
-
 var narensis C83
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Storage Stability

Storage Stability Organism
-4°C, pH 7.5, 20% sucrose solution, 3 week, 20% loss of activity Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-methyl-L-alanine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide Pseudomonas putida L-alanine + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-leucine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide Pseudomonas putida L-leucine + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-valine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide Pseudomonas putida L-valine + formaldehyde + reduced acceptor
-
 ?
N-methylglycine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide Pseudomonas putida glycine + formaldehyde + reduced acceptor
-
 ?
sarcosine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate Pseudomonas putida glycine + formaldehyde + reduced acceptor
-
 ?
sarcosine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate Corynebacterium sp. glycine + formaldehyde + reduced acceptor
-
 ?
sarcosine + H2O + phenazine methosulfate
-
 Pseudomonas putida glycine + formaldehyde + reduced phenazine methosulfate
-
 r
sarcosine + H2O + potassium ferricyanide
-
 Pseudomonas putida glycine + formaldehyde + potassium ferrocyanide
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 42500, SDS-PAGE Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 sarcosine + phenazine methosulfate Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
 not active below, sarcosine + phenazine methosulfate Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9.5 at 20°C for 20 h Pseudomonas putida