Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant T442G enzymes in Escherichia coli strain Bl21(DE3) | Methylophilus methylotrophus |
Protein Variants | Comment | Organism |
---|---|---|
Y442G | site-directed mutagenesis | Methylophilus methylotrophus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, dissociation constants, thermodynamics | Methylophilus methylotrophus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophilus methylotrophus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme, and recombinant wild-type and mutant T442G enzymes expressed in Escherichia coli strain BL21(DE3) | Methylophilus methylotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine + H2O + oxidized electron-transferring flavoprotein | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + reduced electron-transferring flavoprotein | - |
r | |
trimethylamine + H2O + semiquinone electron-transferring flavoprotein | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + reduced electron-transferring flavoprotein | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TMADH | - |
Methylophilus methylotrophus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Abz-VAA | - |
Methylophilus methylotrophus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
electron-transferring flavoprotein | interaction with the enzyme, binding study, reaction mechanism and kinetics | Methylophilus methylotrophus | |
FAD | - |
Methylophilus methylotrophus |