Literature summary for 1.5.8.2 extracted from

Wilson, E.K.; Huang, L.; Sutcliffe, M.J.; Mathews, F.S.; Hille, R.; Scrutton, N.S.
An exposed tyrosine on the surface of trimethylamine dehydrogenase facilitates electron transfer of electron transferring flavoprotein: Kinetics of transfer in wild-type and mutant complexes (1997), Biochemistry, 36, 41-48.

Search for more literature for 1.5.8.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Methylophilus methylotrophus

Protein Variants

Protein Variants	Comment	Organism
Y442F	1.4fold reduction of electron transfer	Methylophilus methylotrophus
Y442G	30.5fold reduction of electron transfer	Methylophilus methylotrophus
Y442L	2.2fold reduction of electron transfer	Methylophilus methylotrophus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0143	-	trimethylamine	mutant Y442F, electron acceptor: electron transferring flavoprotein	Methylophilus methylotrophus
0.0175	-	trimethylamine	wild-type form, electron acceptor: electron transferring flavoprotein	Methylophilus methylotrophus
0.0196	-	trimethylamine	mutant Y442L, electron acceptor: electron transferring flavoprotein	Methylophilus methylotrophus
0.0262	-	trimethylamine	mutant Y442G, electron acceptor: electron transferring flavoprotein	Methylophilus methylotrophus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	4Fe-4S-center	Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
trimethylamine + H2O + electron transferring flavoprotein	Methylophilus methylotrophus	-	dimethylamine + formaldehyde + reduced electron transferring flavoprotein	-	?
trimethylamine + H2O + electron transferring flavoprotein	Methylophilus methylotrophus W3A1	-	dimethylamine + formaldehyde + reduced electron transferring flavoprotein	-	?

Organism

Organism	UniProt	Comment	Textmining
Methylophilus methylotrophus	-	-	-
Methylophilus methylotrophus W3A1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity, chromatography steps	Methylophilus methylotrophus
to homogeneity, recombinant enzyme	Methylophilus methylotrophus

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Methylophilus methylotrophus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
trimethylamine + H2O + electron transferring flavoprotein	-	Methylophilus methylotrophus	dimethylamine + formaldehyde + reduced electron transferring flavoprotein	-	?
trimethylamine + H2O + electron transferring flavoprotein	-	Methylophilus methylotrophus W3A1	dimethylamine + formaldehyde + reduced electron transferring flavoprotein	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.25	-	trimethylamine	mutant Y442G, electron acceptor: electron transferring flavoprotein	Methylophilus methylotrophus
3.8	-	trimethylamine	mutant Y442L, electron acceptor: electron transferring flavoprotein	Methylophilus methylotrophus
10.3	-	trimethylamine	mutant Y442F, electron acceptor: electron transferring flavoprotein	Methylophilus methylotrophus
16.5	-	trimethylamine	wild-type form, electron acceptor: electron transferring flavoprotein	Methylophilus methylotrophus

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Methylophilus methylotrophus

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Release 2023.1 (January 2023)