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Literature summary for 1.5.8.2 extracted from

  • Scrutton, N.S.; Packman, L.C.; Mathews, F.S.; Rohlfs, R.J.; Hille, R.
    Assembly of redox centers in the trimethylamine dehydrogenase of bacterium W3A1. Properties of the wild-type enzyme and a C30A mutant expressed from a cloned gene in Escherichia coli (1994), J. Biol. Chem., 269, 13942-13950.
    View publication on PubMed
Search for more literature for 1.5.8.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methylophilus methylotrophus

Protein Variants

Protein Variants Comment Organism
C30A binds FMN non-covalently Methylophilus methylotrophus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 trimethylamine electron acceptor: ferricenium hexafluorophosphate, wild-type enzyme Methylophilus methylotrophus
0.019
-
 trimethylamine electron acceptor: ferricenium hexafluorophosphate, recombinant enzyme Methylophilus methylotrophus
2
-
 trimethylamine electron acceptor: ferricenium hexafluorophosphate, mutant C30A Methylophilus methylotrophus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 4Fe-4S-center Methylophilus methylotrophus

Organism

Organism UniProt Comment Textmining
Methylophilus methylotrophus
-
-
-
Methylophilus methylotrophus W3A1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Methylophilus methylotrophus
to homogeneity, recombinant enzyme Methylophilus methylotrophus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Methylophilus methylotrophus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trimethylamine + H2O + ferricenium hexafluorophosphate
-
 Methylophilus methylotrophus dimethylamine + formaldehyde + ferrocenium hexafluorophosphate
-
 ?
trimethylamine + H2O + ferricenium hexafluorophosphate
-
 Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + ferrocenium hexafluorophosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17
-
 trimethylamine mutant C30A, electron acceptor: ferricenium hexafluorophosphate Methylophilus methylotrophus
38
-
 trimethylamine electron acceptor: ferricenium hexafluorophosphate Methylophilus methylotrophus
40
-
 trimethylamine recombinant enzyme, electron acceptor: ferricenium hexafluorophosphate Methylophilus methylotrophus

Cofactor

Cofactor Comment Organism Structure
ADP
-
 Methylophilus methylotrophus
FMN covalently bound to Cys30 Methylophilus methylotrophus