Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methylophilus methylotrophus |
Protein Variants | Comment | Organism |
---|---|---|
C30A | removal of the 6-S-cysteinyl-FMN bond, diminishes limiting rate for the first of three observed kinetic phases | Methylophilus methylotrophus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 4Fe-4S-center | Methylophilus methylotrophus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diethylmethylamine + H2O + electron acceptor | Methylophilus methylotrophus | slow substrate, triphasic reaction kinetic | diethylamine + formaldehyde + reduced electron acceptor | - |
? | |
diethylmethylamine + H2O + electron acceptor | Methylophilus methylotrophus W3A1 | slow substrate, triphasic reaction kinetic | diethylamine + formaldehyde + reduced electron acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophilus methylotrophus | - |
- |
- |
Methylophilus methylotrophus W3A1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, recombinant enzyme | Methylophilus methylotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diethylmethylamine + H2O + electron acceptor | slow substrate, triphasic reaction kinetic | Methylophilus methylotrophus | diethylamine + formaldehyde + reduced electron acceptor | - |
? | |
diethylmethylamine + H2O + electron acceptor | slow substrate, triphasic reaction kinetic | Methylophilus methylotrophus W3A1 | diethylamine + formaldehyde + reduced electron acceptor | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | covalently bound to Cys30 | Methylophilus methylotrophus |