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Literature summary for 1.5.5.3 extracted from

  • Summitt, C.B.; Johnson, L.C.; Joensson, T.J.; Parsonage, D.; Holmes, R.P.; Lowther, W.T.
    Proline dehydrogenase 2 (PRODH2) is a hydroxyproline dehydrogenase (HYPDH) and molecular target for treating primary hyperoxaluria (2015), Biochem. J., 466, 273-281 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.5.3

Application

Application Comment Organism
medicine the enzyme is a therapeutic target for the treatment of primary hyperoxaluria Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(1R,3R)-3-hydroxy-cyclopentane-carboxylate
-
 Homo sapiens
5-hydroxy-1H-pyrazole-3-carboxylate
-
 Homo sapiens
menadione
-
 Homo sapiens
additional information (1S,3R)-3-hydroxy-cyclopentane carboxylic acid, trans-4-hydroxy-D-proline, cis-4-hydroxy-L-proline, and cis-4-hydroxy-D-proline do not cause inhibition even up to 5 mM Homo sapiens
Tetrahydrofuroic acid
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 menaquinone at pH 7.5 and 25°C Homo sapiens
0.124
-
 coenzyme Q1 at pH 7.5 and 25°C Homo sapiens
0.143
-
 duroquinone at pH 7.5 and 25°C Homo sapiens
200
-
 trans-4-hydroxy-L-proline at pH 7.5 and 25°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans-4-hydroxy-L-proline + coenzyme Q10 Homo sapiens preferred substrate (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UF12
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and HisPur cobalt resin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline + coenzyme Q1
-
 Homo sapiens ?
-
 ?
additional information no activity with trans-4-hydroxy-D-proline, cis-4-hydroxy-L-proline, and cis-4-hydroxy-D-proline Homo sapiens ?
-
 ?
additional information the enzyme is a poor oxidase using O2 as electron acceptor Homo sapiens ?
-
 ?
trans-4-hydroxy-L-proline + coenzyme Q1
-
 Homo sapiens (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ?
-
 ?
trans-4-hydroxy-L-proline + coenzyme Q10 preferred substrate Homo sapiens (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ?
-
 ?
trans-4-hydroxy-L-proline + duroquinone
-
 Homo sapiens (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + duroquinol
-
 ?
trans-4-hydroxy-L-proline + menadione
-
 Homo sapiens (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + menadiol
-
 ?
trans-4-hydroxy-L-proline + menaquinone
-
 Homo sapiens (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + menaquinol
-
 ?

Synonyms

Synonyms Comment Organism
hydroxyproline oxidase
-
 Homo sapiens
HYPDH
-
 Homo sapiens
ProDH2
-
 Homo sapiens
proline dehydrogenase 2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.19
-
 coenzyme Q1 at pH 7.5 and 25°C Homo sapiens
0.19
-
 trans-4-hydroxy-L-proline at pH 7.5 and 25°C Homo sapiens
0.27
-
 duroquinone at pH 7.5 and 25°C Homo sapiens
0.28
-
 menaquinone at pH 7.5 and 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.306
-
 menadione at pH 7.5 and 25°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.5
-
 at pH 7.5 and 25°C Homo sapiens Tetrahydrofuroic acid
2.1
-
 at pH 7.5 and 25°C Homo sapiens (1R,3R)-3-hydroxy-cyclopentane-carboxylate
2.9
-
 at pH 7.5 and 25°C Homo sapiens 5-hydroxy-1H-pyrazole-3-carboxylate

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.93
-
 trans-4-hydroxy-L-proline at pH 7.5 and 25°C Homo sapiens
1.5
-
 coenzyme Q1 at pH 7.5 and 25°C Homo sapiens
1.9
-
 duroquinone at pH 7.5 and 25°C Homo sapiens
11
-
 menaquinone at pH 7.5 and 25°C Homo sapiens
75
-
 L-proline at pH 7.5 and 25°C Homo sapiens