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Literature summary for 1.5.5.2 extracted from

  • Serrano, H.; Blanchard, J.S.
    Kinetic and isotopic characterization of L-proline dehydrogenase from Mycobacterium tuberculosis (2013), Biochemistry, 52, 5009-5015.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.5.2

Protein Variants

Protein Variants Comment Organism
K110A inactive Mycobacterium tuberculosis
Y203F the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
 2,6-dichlorophenolindophenol wild type enzyme, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis
0.86
-
 L-proline mutant enzyme Y203F, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis
5.7
-
 L-proline wild type enzyme, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 2 * 37000, SDS-PAGE Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline + 2,6-dichlorophenolindophenol + H2O
-
 Mycobacterium tuberculosis DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 37000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
L-proline dehydrogenase
-
 Mycobacterium tuberculosis
PRODH
-
 Mycobacterium tuberculosis
prub
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33.5
-
 L-proline wild type enzyme, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis
100.6
-
 L-proline mutant enzyme Y203F, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.117
-
 L-proline mutant enzyme Y203F, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis
5.877
-
 L-proline wild type enzyme, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis