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Literature summary for 1.5.5.2 extracted from

  • Zhu, W.; Becker, D.F.
    Exploring the proline-dependent conformational change in the multifunctional PutA flavoprotein by tryptophan fluorescence spectroscopy (2005), Biochemistry, 44, 12297-12306.
    View publication on PubMed
Search for more literature for 1.5.5.2

Protein Variants

Protein Variants Comment Organism
additional information truncated enzyme containing residues 86-601 and only four Trp residues. Substantial conformational changes of truncated protein upon addition of proline Escherichia coli
W194F mutation in truncated enzyme containing residues 86-601, ratio of kcat to Km-value drops by 50% Escherichia coli
W194F/W211F mutation in truncated enzyme containing residues 86-601, ratio of kcat to Km-value drops by 95% Escherichia coli
W211F mutation in truncated enzyme containing residues 86-601, ratio of kcat to Km-value drops by 80% Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
32
-
 L-proline truncated mutant W194F/W211F, pH 7.5, 25°C Escherichia coli
56
-
 L-proline truncated mutant W194F, pH 7.5, 25°C Escherichia coli
58
-
 L-proline truncated wild-type, pH 7.5, 25°C Escherichia coli
59
-
 L-proline truncated mutant W211F, pH 7.5, 25°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 enzyme switches from a cytosolic DNA-binding protein to a membrane-bound enzyme with proline dehydrogenase activity and DELTA1-pyrroline-5-carboxylate dehydrogenase activity
-

Reaction

Reaction Comment Organism Reaction ID
L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol switching of enzyme from a cytosolic DNA-binding protein to a membrane-bound enzyme involves W211 in a flexible domain near the proline dehydrogenase active site and occurs in a time scale 10fold lower than the turnover number Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline + FAD + H2O
-
 Escherichia coli (S)-1-pyrroline-5-carboxylate + FADH2
-
 ?

Synonyms

Synonyms Comment Organism
PutA
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 L-proline truncated mutant W194F/W211F, pH 7.5, 25°C Escherichia coli
4
-
 L-proline truncated mutant W211F, pH 7.5, 25°C Escherichia coli
10
-
 L-proline truncated mutant W194F, pH 7.5, 25°C Escherichia coli
20
-
 L-proline truncated wild-type, pH 7.5, 25°C Escherichia coli