Cloned (Comment) | Organism |
---|---|
wild-type PutA86-601 and site-directed mutants | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D370A | mutant form of PutA86-601. The ratio of turnover-number to Km-value is 2.8fold lower than the ratio of the wild-type PutA86-601 | Escherichia coli |
D370A/Y540F | mutant form of PutA86-601. The ratio of turnover-number to Km-value is 15fold lower than the ratio of the wild-type PutA86-601 | Escherichia coli |
Y540F | mutant form of PutA86-601. The ratio of turnover-number to Km-value is 5.8fold lower than the ratio of the wild-type PutA86-601 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
58 | - |
L-proline | pH 8.0, 25°C, wild-type PutA86-601 | Escherichia coli | |
73 | - |
L-proline | pH 8.0, 25°C, D370A mutant of PutA86-601 | Escherichia coli | |
300 | - |
L-proline | pH 8.0, 25°C, Y540F mutant of PutA86-601 | Escherichia coli | |
340 | - |
L-proline | pH 8.0, 25°C, Y540F/D370A mutant of PutA86-601 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type PutA86-601 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline + 2,6-dichlorophenolindophenol + H2O | + phenazine methosulfate as mediator | Escherichia coli | (S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PRODH | - |
Escherichia coli |
PutA flavoprotein | combines DNA-binding, proline dehydrogenase and DELTA1-pyrroline-5-carboxylate dehydrogenase activities | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
L-proline | pH 8.0, 25°C, Y540F mutant of PutA86-601 | Escherichia coli | |
7.8 | - |
L-proline | pH 8.0, 25°C, Y540F/D370A mutant of PutA86-601 | Escherichia coli | |
9.7 | - |
L-proline | pH 8.0, 25°C, D370A mutant of PutA86-601 | Escherichia coli | |
20 | - |
L-proline | pH 8.0, 25°C, wild-type PutA86-601 | Escherichia coli |