Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.5.2 extracted from

  • Baban, B.A.; Vinod, M.P.; Tanner, J.J.; Becker, D.F.
    Probing a hydrogen bond pair and the FAD redox properties in the proline dehydrogenase domain of Escherichia coli PutA (2004), Biochim. Biophys. Acta, 1701, 49-59.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type PutA86-601 and site-directed mutants Escherichia coli

Protein Variants

Protein Variants Comment Organism
D370A mutant form of PutA86-601. The ratio of turnover-number to Km-value is 2.8fold lower than the ratio of the wild-type PutA86-601 Escherichia coli
D370A/Y540F mutant form of PutA86-601. The ratio of turnover-number to Km-value is 15fold lower than the ratio of the wild-type PutA86-601 Escherichia coli
Y540F mutant form of PutA86-601. The ratio of turnover-number to Km-value is 5.8fold lower than the ratio of the wild-type PutA86-601 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
58
-
L-proline pH 8.0, 25°C, wild-type PutA86-601 Escherichia coli
73
-
L-proline pH 8.0, 25°C, D370A mutant of PutA86-601 Escherichia coli
300
-
L-proline pH 8.0, 25°C, Y540F mutant of PutA86-601 Escherichia coli
340
-
L-proline pH 8.0, 25°C, Y540F/D370A mutant of PutA86-601 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
wild-type PutA86-601
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline + 2,6-dichlorophenolindophenol + H2O + phenazine methosulfate as mediator Escherichia coli (S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
?

Synonyms

Synonyms Comment Organism
PRODH
-
Escherichia coli
PutA flavoprotein combines DNA-binding, proline dehydrogenase and DELTA1-pyrroline-5-carboxylate dehydrogenase activities Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
L-proline pH 8.0, 25°C, Y540F mutant of PutA86-601 Escherichia coli
7.8
-
L-proline pH 8.0, 25°C, Y540F/D370A mutant of PutA86-601 Escherichia coli
9.7
-
L-proline pH 8.0, 25°C, D370A mutant of PutA86-601 Escherichia coli
20
-
L-proline pH 8.0, 25°C, wild-type PutA86-601 Escherichia coli