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Literature summary for 1.5.3.6 extracted from
- Binding of FAD to 6-hydroxy-D-nicotine oxidase apoenzyme prevents degradation of the holoenzyme (1989), Biochem. J., 258, 187-192.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphoenolpyruvate | activation of autocatalytical flavinylation, together with FAD protects the enzyme of proteolytic cleavage | Paenarthrobacter nicotinovorans |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JM109 | Paenarthrobacter nicotinovorans |
| expression is temperature dependent, higher specific activities are found at 30°C than at 37°C | Paenarthrobacter nicotinovorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-6-hydroxynicotine + H2O + O2 | Paenarthrobacter nicotinovorans | - |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenarthrobacter nicotinovorans | - |
formerly Arthrobacter oxidans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-6-hydroxynicotine + H2O + O2 | - |
Paenarthrobacter nicotinovorans | 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
denaturation of the holoenzyme | Paenarthrobacter nicotinovorans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Paenarthrobacter nicotinovorans | |
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