Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli HB101 as a 6-hydroxy-D-nicotine oxidase/glutathione S-transferase fusion protein | Paenarthrobacter nicotinovorans |
Crystallization (Comment) | Organism |
---|---|
fusion protein with glutathione S-transferase | Paenarthrobacter nicotinovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-hydroxy-L-nicotine | competitive inhibitor | Paenarthrobacter nicotinovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
6-hydroxy-D-nicotine | - |
Paenarthrobacter nicotinovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48980 | - |
with FAD, amino acid analysis | Paenarthrobacter nicotinovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-6-hydroxynicotine + H2O + O2 | Paenarthrobacter nicotinovorans | - |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | transcient product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter nicotinovorans | - |
strain DSM 420 | - |
Purification (Comment) | Organism |
---|---|
- |
Paenarthrobacter nicotinovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Paenarthrobacter nicotinovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-6-hydroxynicotine + H2O + O2 | - |
Paenarthrobacter nicotinovorans | 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | transcient product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Paenarthrobacter nicotinovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound to a histidine | Paenarthrobacter nicotinovorans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
6-hydroxy-L-nicotine | - |
Paenarthrobacter nicotinovorans |