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Literature summary for 1.5.3.6 extracted from

  • Schenk, S.; Hoelz, A.; Krauss, B.; Decker, K.
    Gene structures and properties of enzymes of the plasmid-encoded nicotine catabolism of Arthrobacter nicotinovorans (1998), J. Mol. Biol., 284, 1323-1339.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli HB101 as a 6-hydroxy-D-nicotine oxidase/glutathione S-transferase fusion protein Paenarthrobacter nicotinovorans

Crystallization (Commentary)

Crystallization (Comment) Organism
fusion protein with glutathione S-transferase Paenarthrobacter nicotinovorans

Inhibitors

Inhibitors Comment Organism Structure
6-hydroxy-L-nicotine competitive inhibitor Paenarthrobacter nicotinovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
6-hydroxy-D-nicotine
-
Paenarthrobacter nicotinovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48980
-
with FAD, amino acid analysis Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 transcient product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously ?

Organism

Organism UniProt Comment Textmining
Paenarthrobacter nicotinovorans
-
strain DSM 420
-

Purification (Commentary)

Purification (Comment) Organism
-
Paenarthrobacter nicotinovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32
-
-
Paenarthrobacter nicotinovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-6-hydroxynicotine + H2O + O2
-
Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 transcient product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously ?

Subunits

Subunits Comment Organism
monomer
-
Paenarthrobacter nicotinovorans

Cofactor

Cofactor Comment Organism Structure
FAD covalently bound to a histidine Paenarthrobacter nicotinovorans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
6-hydroxy-L-nicotine
-
Paenarthrobacter nicotinovorans