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Literature summary for 1.5.3.16 extracted from

  • Fincato, P.; Moschou, P.N.; Spedaletti, V.; Tavazza, R.; Angelini, R.; Federico, R.; Roubelakis-Angelakis, K.A.; Tavladoraki, P.
    Functional diversity inside the Arabidopsis polyamine oxidase gene family (2011), J. Exp. Bot., 62, 1155-1168.
    View publication on PubMed
Search for more literature for 1.5.3.16

Cloned(Commentary)

Cloned (Comment) Organism
AtPAO1, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli, expression of GFP-tagged AtPAO3 in Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
guazatine
-
 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Arabidopsis thaliana
0.12
-
 spermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
0.47
-
 N1-acetylspermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
5.7
-
 thermospermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
6.9
-
 norspermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
spermine + O2 + H2O Arabidopsis thaliana
-
 spermidine + 3-aminopropanal + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Columbia-0
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no oxidation activity with spermidine Arabidopsis thaliana ?
-
 ?
N1-acetylspermidine + O2 + H2O
-
 Arabidopsis thaliana putrescine + 3-acetamidopropanal + H2O2
-
 ?
N1-acetylspermine + O2 + H2O
-
 Arabidopsis thaliana spermidine + 3-acetamidopropanal + H2O2
-
 ?
norspermine + O2 + H2O
-
 Arabidopsis thaliana ?
-
 ?
spermine + O2 + H2O
-
 Arabidopsis thaliana spermidine + 3-aminopropanal + H2O2
-
 ?
thermospermine + O2 + H2O
-
 Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
AtPAO1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 thermospermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
0.09
-
 norspermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
0.2
-
 N1-acetylspermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
2.5
-
 spermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Arabidopsis thaliana

General Information

General Information Comment Organism
evolution AtPAO2-AtPAO4 form a subfamily of polyamine oxidases different from AtPAO1, overview Arabidopsis thaliana