Cloned (Comment) | Organism |
---|---|
SMO/PAOh1 cDNA is subcloned into the pET15b bacterial expression vector, expression in Escherichia coli. NCI-H157 human non-small cell lung carcinoma cells are stably transfected, and individual clones selected that overexpress the enzyme | Homo sapiens |
SMO5 cDNA is subcloned into the pET15b bacterial expression vector, expression in Escherichia coli. NCI-H157 human non-small cell lung carcinoma cells are stably transfected, and individual clones selected that overexpress the enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
spermine | splice variant SMO5 | Homo sapiens | |
0.0006 | - |
spermine | SMO/PAOh1 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | SMO/PAOh1 | Homo sapiens | 5829 | - |
cytosol | SMO5 | Homo sapiens | 5829 | - |
nucleus | SMO/PAOh1 | Homo sapiens | 5634 | - |
nucleus | SMO5 | Homo sapiens | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61900 | - |
x * 61900, SMO/PAOh1, SDS-PAGE | Homo sapiens |
65000 | - |
x * 65000, SMO5, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | Q9NWM0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, SMO/PAOh1 | Homo sapiens |
recombinant enzyme, SMO5 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | SMO/PAOh1 exhibits no oxidase activity when using N1,N12-diacetylspermine, N1-acetylspermidine, N8-acetylspermidine, spermidine, or the polyamine analogues, bis(ethyl)norspermine or N1-ethyl-N11-(cyclopropyl)methyl-4,8,diazaundecane | Homo sapiens | ? | - |
? | |
additional information | splice variant SMO5 exhibits no oxidase activity when using N1,N12-diacetylspermine, N1-acetylspermidine, N8-acetylspermidine, spermidine, or the polyamine analogues, bis(ethyl)norspermine or N1-ethyl-N11-(cyclopropyl)methyl-4,8,diazaundecane | Homo sapiens | ? | - |
? | |
N1-acetylspermine + O2 + H2O | less than 10% of the activity with spermine, SMO/PAOh1 | Homo sapiens | ? | - |
? | |
N1-acetylspermine + O2 + H2O | less than 10% of the activity with spermine, splice variant SMO5 | Homo sapiens | ? | - |
? | |
spermine + O2 + H2O | exhibits a strong preference for spermine as the primary substrate over all other naturally occurring polyamines, SMO/PAOh1 | Homo sapiens | ? | - |
? | |
spermine + O2 + H2O | exhibits a strong preference for spermine as the primary substrate over all other naturally occurring polyamines, splice variant SMO5 | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61900, SMO/PAOh1, SDS-PAGE | Homo sapiens |
? | x * 65000, SMO5, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
SMO/PAOh1 | - |
Homo sapiens |
SMO5 | splice variant protein | Homo sapiens |
spermine oxidase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
N1-acetylspermine | SMO/PAOh1 | Homo sapiens | |
0.28 | - |
N1-acetylspermine | splice variant SMO5 | Homo sapiens | |
3.11 | - |
spermine | splice variant SMO5 | Homo sapiens | |
7.55 | - |
spermine | SMO/PAOh1 | Homo sapiens |