Cloned (Comment) | Organism |
---|---|
expression of mutant mSMOmuDELTA (with a deletion of the nuclear domain) in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | purified mutant mSMOmuDELTA (with a deletion of the nuclear domain A) exhibits biochemical properties very similar to that of mSMOalpha and mSMOmu, in particular a pH optimum of 8.5 in 0.1 m Na-phosphate buffer, a Km value of 0.222 mM and a kcat value of 0.0208 per sec. Removal of the nuclear domain amino acid region abolishes proper nuclear targeting of the mSMOmu isoform | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
spermine | pH 8.5, mutant mSMOmuDELTA (with a deletion of the nuclear domain A) | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | splice variant mSMOmu is present in both nuclear and cytoplasmic compartments, two short protein domains are responsible for the nuclear localization of splice variant mSMOmu | Mus musculus | 5829 | - |
nucleus | splice variant mSMOmu is present in both nuclear and cytoplasmic compartments, two short protein domains are responsible for the nuclear localization of splice variant mSMOmu | Mus musculus | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q99K82 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinantly produced mSMOmuDELTA (with a deletion of the nuclear domain) | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
spermine + O2 + H2O | mutant mSMOmuDELTA (with a deletion of the nuclear domain A) is not active on spermidine, N1-acetylspermidine or N1-acetylspermine | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mSMOmu | splice variant | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0208 | - |
spermine | pH 8.5, mutant mSMOmuDELTA (with a deletion of the nuclear domain A) | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
mutant mSMOmuDELTA (with a deletion of the nuclear domain) | Mus musculus |