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Literature summary for 1.5.3.14 extracted from

  • Liu, T.; Kim, D.W.; Niitsu, M.; Maeda, S.; Watanabe, M.; Kamio, Y.; Berberich, T.; Kusano, T.
    Polyamine oxidase 7 is a terminal catabolism-type enzyme in Oryza sativa and is specifically expressed in anthers (2014), Plant Cell Physiol., 55, 1110-1122 .
    View publication on PubMed
Search for more literature for 1.5.3.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0383
-
 spermine pH 6.5, 30°C Oryza sativa
0.0407
-
 spermine pH 7.0, 37°C Oryza sativa
0.0422
-
 spermidine pH 7.0, 37°C Oryza sativa
0.045
-
 spermidine pH 6.5, 30°C Oryza sativa
0.0594
-
 N1-acetylspermine pH 6.5, 30°C Oryza sativa
0.0701
-
 N1-acetylspermine pH 7.0, 37°C Oryza sativa
0.075
-
 thermospermine pH 6.5, 30°C Oryza sativa
0.0788
-
 norspermine pH 6.5, 30°C Oryza sativa
0.0803
-
 N1-acetylspermidine pH 7.0, 37°C Oryza sativa
0.081
-
 N1-acetylspermidine pH 6.5, 30°C Oryza sativa
0.0814
-
 thermospermine pH 7.0, 37°C Oryza sativa
0.0829
-
 norspermine pH 7.0, 37°C Oryza sativa
0.109
-
 norspermidine pH 7.0, 37°C Oryza sativa
0.1578
-
 norspermidine pH 6.5, 30°C Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
apoplast OsPAO7 seems to remain in a peripheral layer of the plant cell with the aid of its predicted signal peptide and transmembrane domain Oryza sativa 48046
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 gel filtration Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q0J290
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
anther the enzyme is specifically expressed in anthers, with an expressional peak at the bicellular pollen stage Oryza sativa
-
pollen the enzyme is specifically expressed in anthers, with an expressional peak at the bicellular pollen stage Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with the diamines putrescine and cadaverine Oryza sativa ?
-
 ?
N1-acetylspermidine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa 1,3-diaminopropane + H2O2 + ?
-
 ?
N1-acetylspermine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa 1,3-diaminopropane + H2O2 + ?
-
 ?
norspermidine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa 1,3-diaminopropane + H2O2 + ?
-
 ?
norspermine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa ?
-
 ?
spermidine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa 4-aminobutanal + propane-1,3-diamine + H2O2
-
 ?
spermine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa N-(3-aminopropyl)-4-aminobutanal + 1,3-diaminopropane + H2O2
-
 ?
thermospermine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa 1,3-diaminopropane + H2O2 + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Oryza sativa
monomer 1 * 53200, calculated from sequence Oryza sativa

Synonyms

Synonyms Comment Organism
OsPAO7
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 substrate: spermine Oryza sativa
37
-
 substrate: spermidine Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 norspermidine pH 6.5, 30°C Oryza sativa
2.3
-
 norspermidine pH 7.0, 37°C Oryza sativa
4.5
-
 thermospermine pH 7.0, 37°C Oryza sativa
6.6
-
 N1-acetylspermidine pH 6.5, 30°C Oryza sativa
7.8
-
 thermospermine pH 6.5, 30°C Oryza sativa
8.3
-
 norspermine pH 7.0, 37°C Oryza sativa
9
-
 norspermine pH 6.5, 30°C Oryza sativa
9.8
-
 N1-acetylspermidine pH 7.0, 37°C Oryza sativa
18.2
-
 N1-acetylspermine pH 7.0, 37°C Oryza sativa
20.5
-
 N1-acetylspermine pH 6.5, 30°C Oryza sativa
26.5
-
 spermidine pH 6.5, 30°C Oryza sativa
30.6
-
 spermine pH 7.0, 37°C Oryza sativa
32.6
-
 spermine pH 6.5, 30°C Oryza sativa
287.9
-
 spermidine pH 7.0, 37°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 substrate: spermine Oryza sativa
7
-
 substrate: spermidine Oryza sativa

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Oryza sativa

General Information

General Information Comment Organism
physiological function a likely role for OsPAO7 during anther development is to deliver H2O2 for secondary wall thickening through lignin formation Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.97
-
 norspermidine pH 6.5, 30°C Oryza sativa
21.7
-
 norspermidine pH 7.0, 37°C Oryza sativa
55.3
-
 thermospermine pH 7.0, 37°C Oryza sativa
82.7
-
 N1-acetylspermidine pH 6.5, 30°C Oryza sativa
96.9
-
 norspermine pH 7.0, 37°C Oryza sativa
104
-
 thermospermine pH 6.5, 30°C Oryza sativa
115.1
-
 norspermine pH 6.5, 30°C Oryza sativa
117.8
-
 N1-acetylspermidine pH 7.0, 37°C Oryza sativa
259.7
-
 N1-acetylspermine pH 7.0, 37°C Oryza sativa
345.1
-
 N1-acetylspermine pH 6.5, 30°C Oryza sativa
591.7
-
 spermidine pH 6.5, 30°C Oryza sativa
660.9
-
 spermidine pH 7.0, 37°C Oryza sativa
752.9
-
 spermine pH 7.0, 37°C Oryza sativa
853.9
-
 spermine pH 6.5, 30°C Oryza sativa