Crystallization (Comment) | Organism |
---|---|
crystallised by the hanging drop vapour-diffusion method, with the protein solution consisting of 5 mg enzyme/ml in 300 mM NaCl and 50 mM sodium phosphate buffer, pH 6.0. Crystal structure of polyamine oxidase is determined to a resolution of 1.9 A. The enzyme contains two domains, which define a remarkable 30 A long U-shaped catalytic tunnel at their interface. The structure of PAO in complex with the inhibitor MDL72527 reveals the residues forming the catalytic machinery and unusual enzyme-inhibitor CH...OH bonds. A ring of glutamate and aspartate residues surrounding one of the two tunnel openings contributes to the steering of the substrate towards the inside of the tunnel | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N,N'-bis(2,3-butadienyl)-1,4-butane-diamine | i.e. MDL72527 | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | O64411 | - |
- |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the FAD prosthetic group is non-covalently bound to the protein and is deeply embedded within the structure. All FAD atoms are solvent-inaccessible, with the exception of the flavin C5a, N5 and C4a atoms that line the active centre | Zea mays |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00055 | - |
N,N'-bis(2,3-butadienyl)-1,4-butane-diamine | pH 6.5, 25°C | Zea mays |