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Literature summary for 1.5.3.13 extracted from
- Identification and biochemical characterization of polyamine oxidases in amphioxus Implications for emergence of vertebrate-specific spermine and acetylpolyamine oxidases (2016), Gene, 575, 429-437 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type and mutant enzymes expressed in Escherichia coli | Branchiostoma japonicum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H69E | compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value | Branchiostoma japonicum |
| H69Q | compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value | Branchiostoma japonicum |
| K315M | compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value | Branchiostoma japonicum |
| T467S | compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value | Branchiostoma japonicum |
| T467Y | compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value | Branchiostoma japonicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N1,N4-bis(2,3-butadienyl)-1,4-butanediamine | 2.5 mM, 70% inhibition of N1-acetylspermine oxidation | Branchiostoma japonicum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0369 | - |
N1-acetylspermine | 37°C, pH 8.0, wild-type enzyme | Branchiostoma japonicum | |
| 0.043 | - |
N1-acetylspermine | 37°C, pH 8.0, mutant enzyme K315M | Branchiostoma japonicum | |
| 0.046 | - |
N1-acetylspermine | 37°C, pH 8.0, mutant enzyme T467Y | Branchiostoma japonicum | |
| 0.058 | - |
N1-acetylspermine | 37°C, pH 8.0, mutant enzyme T467S | Branchiostoma japonicum | |
| 0.062 | - |
N1-acetylspermine | 37°C, pH 8.0, mutant enzyme H69E | Branchiostoma japonicum | |
| 0.071 | - |
N1-acetylspermine | 37°C, pH 8.0, mutant enzyme H69Q | Branchiostoma japonicum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Branchiostoma japonicum | A0A0N7IZL9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Branchiostoma japonicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gill | - |
Branchiostoma japonicum | - |
| hepatic cecum | - |
Branchiostoma japonicum | - |
| hindgut | - |
Branchiostoma japonicum | - |
| muscle | - |
Branchiostoma japonicum | - |
| notochord | - |
Branchiostoma japonicum | - |
| ovary | - |
Branchiostoma japonicum | - |
| testis | - |
Branchiostoma japonicum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bjpao2 shows no activity with spermine | Branchiostoma japonicum | ? | - |
? | |
| N1-acetylspermine + O2 + H2O | - |
Branchiostoma japonicum | spermidine + 3-acetamidopropanal + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 59000, SDS-PAGE | Branchiostoma japonicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bjpao2 | - |
Branchiostoma japonicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | - |
Branchiostoma japonicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
substrate: N1-acetylspermine | Branchiostoma japonicum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Branchiostoma japonicum | calculated from sequence | - |
5.13 |
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