Application | Comment | Organism |
---|---|---|
analysis | assay method to measure N1-acetylpolyamine oxidase activity with N1,N11-didansylnorspermine as the substrate by high performance liquid chromatography. Among the dansylpolyamines, N1,N11-didansylnorspermine is a useful substrate for the development of the N1-acetylpolyamine oxidase assay method. Contaminating polyamines in biological samples do not interfere with the method | Rattus norvegicus |
analysis | assay method to measure N1-acetylpolyamine oxidase activity with N1,N11-didansylnorspermine as the substrate by high performance liquid chromatography. Applied to cell extract from Chinese hamster ovary cell samples, the PAO activity can easily be detected with as little as 10 microg of protein, which corresponds to 1105 cells. The method is rapid, sensitive and useful. Contaminating polyamines in biological samples do not interfere with the method | Cricetulus griseus |
Organism | UniProt | Comment | Textmining |
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Cricetulus griseus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N1,N11-didansylnorspermine + O2 + H2O | - |
Cricetulus griseus | 1-dansylnorspermidine + 1-dansylamido-3-propanal + H2O2 | - |
? | |
N1,N11-didansylnorspermine + O2 + H2O | - |
Rattus norvegicus | 1-dansylnorspermidine + 1-dansylamido-3-propanal + H2O2 | - |
? |