Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time quantitative PCR expression analysis, expression in Pichia pastoris | Papaver somniferum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | baculo virus-induced gene silencing in planta using Agrobacterium tumefaciens strain GV3101 transfection to reduce DBOX transcript levels, real-time quantitative PCR expression analysis | Papaver somniferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.146 | - |
(S)-scoulerine | pH 8.8, 37°C | Papaver somniferum | |
0.201 | - |
Dihydrosanguinarine | pH 8.8, 37°C | Papaver somniferum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(RS)-canadine + O2 | Papaver somniferum | - |
berberine + H2O2 | - |
? | |
(RS)-tetrahydrocolumbamine + O2 | Papaver somniferum | - |
? | - |
? | |
(RS)-tetrahydropalmitine + O2 | Papaver somniferum | - |
? | - |
? | |
(RS)-tetrahydropapaverine + O2 | Papaver somniferum | - |
papaverine + H2O2 | - |
? | |
(S)-scoulerine + O2 | Papaver somniferum | DBOX does not fully aromatize the C-ring of (S)-scoulerine but instead catalyzes a two-electron oxidation | ? | - |
? | |
(S)-stylopine + O2 | Papaver somniferum | - |
? | - |
? | |
dihydrochelerythrine + O2 | Papaver somniferum | - |
chelerythine + H2O2 | - |
? | |
dihydrosanguinarine + O2 | Papaver somniferum | best substrate | sanguinarine + H2O2 | - |
? | |
additional information | Papaver somniferum | the recombinant enzyme preferentially catalyzes the oxidation of dihydrosanguinarine to sanguinarine but also converts (RS)-tetrahydropapaverine to papaverine and several protoberberine alkaloids to oxidized forms, including (RS)-canadine to berberine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver somniferum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower bud | very low level compared to roots | Papaver somniferum | - |
leaf | very low level compared to roots | Papaver somniferum | - |
additional information | enzyme expression patterns, overview | Papaver somniferum | - |
root | predominantly | Papaver somniferum | - |
seedling | - |
Papaver somniferum | - |
stem | very low level compared to roots | Papaver somniferum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(RS)-canadine + O2 | - |
Papaver somniferum | berberine + H2O2 | - |
? | |
(RS)-tetrahydrocolumbamine + O2 | - |
Papaver somniferum | ? | - |
? | |
(RS)-tetrahydropalmitine + O2 | - |
Papaver somniferum | ? | - |
? | |
(RS)-tetrahydropapaverine + O2 | - |
Papaver somniferum | papaverine + H2O2 | - |
? | |
(RS)-tetrahydropapaverine + O2 | - |
Papaver somniferum | ? | - |
? | |
(S)-scoulerine + O2 | DBOX does not fully aromatize the C-ring of (S)-scoulerine but instead catalyzes a two-electron oxidation | Papaver somniferum | ? | - |
? | |
(S)-stylopine + O2 | - |
Papaver somniferum | ? | - |
? | |
dihydrochelerythrine + O2 | - |
Papaver somniferum | chelerythine + H2O2 | - |
? | |
dihydrochelerythrine + O2 | - |
Papaver somniferum | ? | - |
? | |
dihydrosanguinarine + O2 | best substrate | Papaver somniferum | sanguinarine + H2O2 | - |
? | |
additional information | substrate specificity, overview | Papaver somniferum | ? | - |
? | |
additional information | the recombinant enzyme preferentially catalyzes the oxidation of dihydrosanguinarine to sanguinarine but also converts (RS)-tetrahydropapaverine to papaverine and several protoberberine alkaloids to oxidized forms, including (RS)-canadine to berberine | Papaver somniferum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DBOX | - |
Papaver somniferum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Papaver somniferum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Papaver somniferum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Papaver somniferum |
General Information | Comment | Organism |
---|---|---|
malfunction | virus-induced gene silencing to reduce DBOX transcript levels results in a corresponding reduction in sanguinarine, dihydrosanguinarine, and papaverine accumulation in opium poppy roots | Papaver somniferum |
metabolism | DBOX as a multifunctional oxidative enzyme in benzylisoquinoline alkaloid metabolism, overview. The two-electron oxidation of dihydrosanguinarine catalyzed by dihydrobenzophenanthridine oxidase is the final step in sanguinarine biosynthesis. The formation of the fully conjugated ring system in sanguinarine is similar to the four-electron oxidations of (S)-canadine to berberine and (S)-tetrahydropapaverine to papaverine | Papaver somniferum |