Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus sp. BSD-8 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. BSD-8 | Q6ITC6 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity-purification, 4-aminopyrrole-2-carboxylic acid as the affinity ligand, which is coupled with Sepharose CL 4B via spacers composed of epichlorohydrin and ethylenediamine. With the affinity medium, the purification process consists of only one affinity chromatography step to capture monomeric enzyme. The purified enzyme iss 94% and 96% pure when analyzed on an HPLC and reducing SDS-PAGE | Bacillus sp. BSD-8 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + H2O + O2 | - |
Bacillus sp. BSD-8 | glycine + formaldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46000, SDS-PAGE | Bacillus sp. BSD-8 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus sp. BSD-8 |