Cloned (Comment) | Organism |
---|---|
mutant proteins expressed in Escherichia coli BL21(DE3) | Corynebacterium sp. U-96 |
Protein Variants | Comment | Organism |
---|---|---|
K171A | 39% activity of the wild-type enzyme, strongly increased Km for oxygen | Corynebacterium sp. U-96 |
K171D | 32% activity of the wild-type enzyme, strongly increased Km for oxygen | Corynebacterium sp. U-96 |
K171R | 58% activity of the wild-type enzyme | Corynebacterium sp. U-96 |
K358A | inactive | Corynebacterium sp. U-96 |
K358D | inactive | Corynebacterium sp. U-96 |
K358R | 0.07% of activity, increased Km | Corynebacterium sp. U-96 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0312 | - |
oxygen | K171R mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
0.0329 | - |
oxygen | wild-type enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
0.386 | - |
oxygen | K171A mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
0.73 | - |
oxygen | K171D mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
1.47 | - |
sarcosine | K171A mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
1.72 | - |
sarcosine | K171R mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
2.75 | - |
sarcosine | K171D mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
2.76 | - |
sarcosine | wild-type enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
17.4 | - |
sarcosine | K358R mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + O2 + H2O | Corynebacterium sp. U-96 | - |
formaldehyde + glycine + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. U-96 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 | mechanism suggested | Corynebacterium sp. U-96 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + O2 + H2O | - |
Corynebacterium sp. U-96 | formaldehyde + glycine + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
monomeric sarcosine oxidase | - |
Corynebacterium sp. U-96 |
MSOX | - |
Corynebacterium sp. U-96 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0113 | - |
sarcosine | K358R mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
2.25 | - |
sarcosine | K171A mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
2.64 | - |
sarcosine | K171D mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
6.1 | - |
oxygen | K171D mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
7.4 | - |
oxygen | K171A mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
9.23 | - |
sarcosine | K171R mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
9.8 | - |
oxygen | K171R mutant enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
18.5 | - |
sarcosine | wild-type enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 | |
18.9 | - |
oxygen | wild-type enzyme, pH 8.0, 25°C | Corynebacterium sp. U-96 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalent and non-covalent | Corynebacterium sp. U-96 | |
FMN | covalent | Corynebacterium sp. U-96 |