Cloned (Comment) | Organism |
---|---|
mutant proteins expressed as His-tag fusion proteins in Escherichia coli BL21(DE3) | Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
K265A | only traces of activity | Bacillus sp. (in: Bacteria) |
K265M | only traces of activity | Bacillus sp. (in: Bacteria) |
K265Q | only traces of activity | Bacillus sp. (in: Bacteria) |
K265R | less than 30% of covalently bound FAD, strongly reduced turnover | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methylthioacetate | competitive | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
1 * 44000 | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + O2 + H2O | Bacillus sp. (in: Bacteria) | - |
formaldehyde + glycine + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant proteins using His-tag | Bacillus sp. (in: Bacteria) |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 | mechanism suggested | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + O2 + H2O | - |
Bacillus sp. (in: Bacteria) | formaldehyde + glycine + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000 | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
monomeric sarcosine oxidase | - |
Bacillus sp. (in: Bacteria) |
MSOX | - |
Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0049 | - |
sarcosine | K265Q mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
0.0066 | - |
sarcosine | K265A mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
0.0076 | - |
sarcosine | K265M mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
0.272 | - |
sarcosine | K265R mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
45.8 | - |
sarcosine | native protein, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound | Bacillus sp. (in: Bacteria) |