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Literature summary for 1.5.3.1 extracted from

  • Guo, K.; Ma, X.; Sun, G.; Zhao, Y.; Li, X.; Zhao, W.; Kai, L.
    Expression and characterization of a thermostable sarcosine oxidase (SOX) from Bacillus sp. in Escherichia coli (2006), Appl. Microbiol. Biotechnol., 73, 559-566.
    View publication on PubMed
Search for more literature for 1.5.3.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1
-
 sarcosine pH 8.5, 60°C Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, calculated, x * 51000, SDS-PAGE Bacillus sp. (in: Bacteria)
51000
-
 x * 43000, calculated, x * 51000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) Q6ITC6 expression in Escherichia coli
-
Bacillus sp. (in: Bacteria) BSD-8 Q6ITC6 expression in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sarcosine + H2O + O2
-
 Bacillus sp. (in: Bacteria) glycine + formaldehyde + H2O2
-
 ?
sarcosine + H2O + O2
-
 Bacillus sp. (in: Bacteria) BSD-8 glycine + formaldehyde + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, calculated, x * 51000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable Bacillus sp. (in: Bacteria)
65
-
 10 min, complete loss of activity Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20.3
-
 sarcosine pH 8.5, 60°C Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 11
-
 Bacillus sp. (in: Bacteria)

Cofactor

Cofactor Comment Organism Structure
FAD wild-type enzyme, noncovalently bound, recombinant enzyme, covalently bound Bacillus sp. (in: Bacteria)