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Literature summary for 1.5.1.8 extracted from

  • Tang, G.; Miron, D.; Zhu-Shimoni, J.X.; Galili, G.
    Regulation of lysine catabolism through lysine-ketoglutarate reductase and saccharopine dehydrogenase in Arabidopsis (1997), Plant Cell, 9, 1305-1316.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.8

Cloned(Commentary)

Cloned (Comment) Organism
cloning of cDNA encoding the bifunctional protein with lysine 2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, expression of the single gene is subject to spatial and developmental controls, expression in Escherichia coli produces no active LKR, perhaps because of a missing post-translational modification in prokaryotes, expression in Saccharomyces cerevisiae mutant lys1 Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
117000
-
 x * 117000, bifunctional protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, calculated from cDNA sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH Saccharomyces cerevisiae plays essential role in lysine biosynthesis, LKR and SDH are separate polypeptides N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH Arabidopsis thaliana first enzyme in lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH Arabidopsis thaliana lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 var. C24
-
Arabidopsis thaliana
-
 ecotype Landsberg erecta and Columbia
-
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information enzyme may be activated by post-translational modification Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Arabidopsis thaliana
-
endosperm outer layers Arabidopsis thaliana
-
flower LKR mRNA is highly abundant in ovules and vascular tissue of anther filaments Arabidopsis thaliana
-
additional information not in pollen grains Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH
-
 Saccharomyces cerevisiae N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Arabidopsis thaliana N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH plays essential role in lysine biosynthesis, LKR and SDH are separate polypeptides Saccharomyces cerevisiae N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH first enzyme in lysine catabolism Arabidopsis thaliana N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH lysine catabolism Arabidopsis thaliana N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
? x * 117000, bifunctional protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, calculated from cDNA sequence Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Arabidopsis thaliana