Protein Variants | Comment | Organism |
---|---|---|
additional information | high-Lys opaque-2 mutant has significantly reduced LKR activity | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Alkaline phosphatase | reduces LKR activity | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
2-oxoglutarate | - |
Glycine max | |
13.7 | - |
L-lysine | - |
Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
123000 | - |
2 * 123000, bifunctional protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, additional 100 kDa band is a proteolytic cleavage product, SDS-PAGE | Glycine max |
256000 | - |
gel filtration, bifunctional protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | Zea mays | - |
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | Oryza sativa | - |
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | Glycine max | first and possibly a rate-limiting step in lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | Glycine max | enzyme activity is modulated in vivo by a lysine-dependent intracellular signaling cascade, mediated by Ca2+ and protein phosphorylation/dephosphorylation | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Oryza sativa | - |
- |
- |
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1630.5fold purification of a bifunctional protein containing lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | developing | Zea mays | - |
seed | developing | Glycine max | - |
seed | developing | Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.261 | - |
- |
Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | - |
Zea mays | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | - |
Oryza sativa | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Zea mays | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Glycine max | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Oryza sativa | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | first and possibly a rate-limiting step in lysine catabolism | Glycine max | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | enzyme activity is modulated in vivo by a lysine-dependent intracellular signaling cascade, mediated by Ca2+ and protein phosphorylation/dephosphorylation | Glycine max | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Zea mays | ? | - |
? | |
additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Glycine max | ? | - |
? | |
additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Oryza sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Zea mays |
homodimer | - |
Oryza sativa |
homodimer | 2 * 123000, bifunctional protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, additional 100 kDa band is a proteolytic cleavage product, SDS-PAGE | Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Zea mays |
30 | - |
assay at | Glycine max |
30 | - |
assay at | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Zea mays | |
NADPH | - |
Glycine max | |
NADPH | - |
Oryza sativa |