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Literature summary for 1.5.1.8 extracted from

  • Gaziola, S.A.; Teixeira, C.M.; Lugli, J.; Sodek, L.; Azevedo, R.A.
    The enzymology of lysine catabolism in rice seeds. Isolation, characterization, and regulatory properties of a lysine 2-oxoglutarate reductase/saccharopine dehydrogenase bifunctional polypeptide (1997), Eur. J. Biochem., 247, 364-371.
    View publication on PubMed
Search for more literature for 1.5.1.8

General Stability

General Stability Organism
presence of 5% w/v insoluble polyvinylpyrrolidone and 10% v/v glycerol in extraction buffer and glycerol in running buffers is crucial to maintain enzyme activity Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
saccharopine product inhibition, competitive with 2-oxoglutarate, noncompetitive with L-lysine Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 NADPH
-
 Oryza sativa
0.79
-
 2-oxoglutarate
-
 Oryza sativa
4.48
-
 L-lysine
-
 Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
202000
-
 non-denaturing PAGE, bifunctional protein containing lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, one band represents the holoenzyme and shows higher enzyme activities compared to a second band of 396 kDa with much lower enzyme activities indicating a multimeric structure Oryza sativa
203000
-
 gel filtration, bifunctional protein containing lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH Oryza sativa regulation of lysine catabolism may be essential for lysine accumulation in rice plants N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH Oryza sativa lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH Oryza sativa IAC 165 regulation of lysine catabolism may be essential for lysine accumulation in rice plants N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH Oryza sativa IAC 165 lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 IAC 165
-
Oryza sativa IAC 165
-
 IAC 165
-

Purification (Commentary)

Purification (Comment) Organism
38fold purification Oryza sativa
partial purification of a single bifunctional protein with lysine-2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa

Reaction

Reaction Comment Organism Reaction ID
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-lysine + 2-oxoglutarate + NADPH + H+ ordered sequence mechanism, where 2-oxoglutarate is first substrate and saccharopine is last product Oryza sativa
a

Source Tissue

Source Tissue Comment Organism Textmining
seed developing Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Oryza sativa N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH regulation of lysine catabolism may be essential for lysine accumulation in rice plants Oryza sativa N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH lysine catabolism Oryza sativa N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Oryza sativa IAC 165 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH regulation of lysine catabolism may be essential for lysine accumulation in rice plants Oryza sativa IAC 165 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH lysine catabolism Oryza sativa IAC 165 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa ?
-
 ?
additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa IAC 165 ?
-
 ?

Subunits

Subunits Comment Organism
More 369 kDa band may represent a dimeric or tetrameric molecule, multimeric form results from the association of the 202 kDa LOR/SDH band Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Oryza sativa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Oryza sativa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2
-
 saccharopine
-
 Oryza sativa