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Literature summary for 1.5.1.8 extracted from

  • Gaziola, S.A.; Sodek, L.; Arruda, P.; Lea, P.J.; Azevedo, R.A.
    Degradation of lysine in rice seeds: Effect of calcium, ionic strength, S-adenosylmethionine and S-2-aminoethyl-L-cysteine on the lysine 2-oxoglutarate reductase-saccharopine dehydrogenase bifunctional enzyme (2000), Physiol. Plant., 110, 164-171.
No PubMed abstract available
Search for more literature for 1.5.1.8

Activating Compound

Activating Compound Comment Organism Structure
organic solvent activates Zea mays

Cloned(Commentary)

Cloned (Comment) Organism
genes encoding bifunctional protein with lysine 2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities are cloned Zea mays
genes encoding bifunctional protein with lysine 2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities are cloned Arabidopsis thaliana

General Stability

General Stability Organism
stabilization of holoenzyme by high salt concentrations, e.g. KCl Zea mays
stabilization of holoenzyme by high salt concentrations, e.g. KCl Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
EGTA 1 mM, 21% loss of activity, addition of 1.6 mM CaCl2 increases activity by 43% Oryza sativa
EGTA up to 70% inhibition, dependent on salt concentration of buffer Zea mays
S-2-aminoethyl-L-cysteine inhibits when present in assay with L-lysine, possibly competitive with L-lysine Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17
-
 S-2-aminoethyl-L-cysteine
-
 Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, lysine 2-oxoglutarate reductase activity is modulated by Ca2+, possible modulation of an inhibitory domain Zea mays
Ca2+ activates, lysine 2-oxoglutarate reductase activity is modulated by Ca2+, possible modulation of an inhibitory domain Oryza sativa
Ca2+ 1.6 mM, activates Oryza sativa
Mg2+ activates Zea mays
Salt activated by high salt concentrations Zea mays
Salt activated by high salt concentrations Oryza sativa
Salt lysine 2-oxoglutarate reductase domain is modulated by ionic strength Zea mays
Salt lysine 2-oxoglutarate reductase domain is modulated by ionic strength Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH Zea mays lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH Arabidopsis thaliana lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH Oryza sativa lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH Oryza sativa IAC 165 lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Oryza sativa
-
 IAC 165
-
Oryza sativa IAC 165
-
 IAC 165
-
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of a single bifunctional protein with lysine-2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
 Arabidopsis thaliana
-
endosperm located specifically in Zea mays
-
endosperm located specifically in Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Zea mays
additional information
-
-
 Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Zea mays N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Arabidopsis thaliana N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Oryza sativa N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH lysine catabolism Zea mays N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH lysine catabolism Arabidopsis thaliana N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH lysine catabolism Oryza sativa N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Oryza sativa IAC 165 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH lysine catabolism Oryza sativa IAC 165 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Zea mays ?
-
 ?
additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Arabidopsis thaliana ?
-
 ?
additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa ?
-
 ?
additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa IAC 165 ?
-
 ?
S-2-aminoethyl-L-cysteine + 2-oxoglutarate + NADPH 52% of activity with equimolar concentration of L-lysine, same Vmax, but higher Km-value than for L-lysine Oryza sativa ?
-
 ?
S-2-aminoethyl-L-cysteine + 2-oxoglutarate + NADPH 52% of activity with equimolar concentration of L-lysine, same Vmax, but higher Km-value than for L-lysine Oryza sativa IAC 165 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information lower turnover number with S-2-aminoethyl-L-cysteine as substrate than with L-lysine Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Zea mays
7
-
-
 Oryza sativa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Zea mays
NADPH
-
 Arabidopsis thaliana
NADPH
-
 Oryza sativa