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Literature summary for 1.5.1.8 extracted from

  • Hutzler, J.; Dancis, J.
    Lysine-ketoglutarate reductase in human tissues (1975), Biochim. Biophys. Acta, 377, 42-51.
    View publication on PubMed
Search for more literature for 1.5.1.8

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, slight activation, effect not consistant Homo sapiens
Triton X-100 1%, slight activation, effect not consistant Homo sapiens

Application

Application Comment Organism
medicine hyperlysinemia: genetic disease, deficiency of lysine-ketoglutarate reductase results in extreme elevations of serum lysine Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 3 mM, 28% loss of activity Homo sapiens
cadaverine 3 mM, 26% loss of activity Homo sapiens
Carbamoylphosphate 3 mM, 25% loss of activity Homo sapiens
CoCl2 1 mM, 82% loss of activity Homo sapiens
CuSO4 1 mM, 87% loss of activity Homo sapiens
DL-pipecolic acid 6 mM, 47% loss of activity Homo sapiens
HgCl2 0.01 mM, 89% loss of activity Homo sapiens
hydroxylamine 3 mM, 33% loss of activity Homo sapiens
L-Glutamic acid 3 mM, 40% loss of activity Homo sapiens
L-Homocitrulline 3 mM, 49% loss of activity Homo sapiens
L-Lysylglycine 3 mM, 25% loss of activity Homo sapiens
L-ornithine 3 mM, 39% loss of activity Homo sapiens
leucine
-
 Homo sapiens
MgSO4 1 mM, 79% loss of activity Homo sapiens
MnCl2 1 mM, 52% loss of activity Homo sapiens
additional information not inhibited by 40 mM ammonium chloride, inhibition by NH4+ is probably due to contamination with glutamic dehydrogenase Homo sapiens
saccharopine 1 mM, 30% inhibition, product inhibition Homo sapiens
ZnCl2 3 mM, complete inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 NADPH
-
 Homo sapiens
1
-
 2-oxoglutarate
-
 Homo sapiens
1.5
-
 L-lysine
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH Homo sapiens saccharopine pathway is the major route of lysine breakdown N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
44.6fold partial purification from liver Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
heart after liver next highest enzyme concentration Homo sapiens
-
kidney low enzyme concentration Homo sapiens
-
liver highest enzyme concentration Homo sapiens
-
additional information trace enzyme activity in muscle, brain, and spleen, no activity in pancreas, adrenal, lung, gut, leucocytes, erythrocytes Homo sapiens
-
skin low enzyme concentration Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 1 month, 50% loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-delta-hydroxylysine + 2-oxoglutarate + NADPH 18% of velocity with equimolar concentration of L-lysine Homo sapiens ?
-
 ?
L-lysine + 2-oxoglutarate + NADPH reverse reaction not detected Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ir
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ir
L-lysine + 2-oxoglutarate + NADPH saccharopine pathway is the major route of lysine breakdown Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
additional information less than 2% of the rate of L-lysine: D-lysine, cadaverine, delta-aminovaleric acid, alpha-acetyl-L-lysine, alpha-amino-epsilon-hydroxycaproic acid, L-lysylglycine, L-2,4-diaminobutyric acid, DL-pipecolic acid, L-ornithine, epsilon-aminocaproic acid, 4% of the rate of L-lysine: DL-alpha,epsilon-diaminopimelic acid, 7% of the rate of 2-oxoglutarate: oxaloacetic acid, less than 2% of the rate of 2-oxoglutarate: 2-oxoadipate, pyruvate, 2-oxoisocaproate, 2-oxoisovalerate, levulinate, 2-hydroxyglutarate Homo sapiens ?
-
 ?
S-2-aminoethyl-L-cysteine + 2-oxoglutarate + NADPH 47% of velocity with equimolar concentration of L-lysine Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 10 min, unstable above Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.2 8.8 about 50% of activity maximum at pH 7.2 and 8.8 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.4
-
 10 min, 50% loss of activity Homo sapiens
4.6 4.9 10 min, stable Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Homo sapiens