Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3)-RIL cells | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
the apo-enzyme is obtained using the hanging drop vapour diffusion method with 100 mM Tris (pH 7.0) and 22% (w/v) PEG 8000, sulfate-, AMP-, and oxalylglycine-bound enzyme crystals are obtained by the hanging drop vapour diffusion method with 26% (w/v) PEG-MME 2000 in 100 mM Tris (pH 7.0) containing 200 mM (NH4)2SO4, or with 24% (w/v) PEG-MME 2000 in 100 mM Bis-Tris, pH 6.5, or with 24% (w/v) PEG-MME 2000 in 100 mM Tris, pH 8.0, at 4°C, respectively | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P38998 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | - |
Saccharomyces cerevisiae | L-lysine + 2-oxoglutarate + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
N6-(glutaryl-2)-L-lysine:nicotinamide adenine dinucleotide (NAD+) oxidoreductase (L-lysine-forming) | - |
Saccharomyces cerevisiae |
saccharopine dehydrogenase (L-lysine-forming) | - |
Saccharomyces cerevisiae |
SDH | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae |